The effect of resveratrol on the migration and proliferation ability of stem cells derived from menstrual blood of women with endometriosis

Subject Areas : cellular , molecular

Hoda Fazaeli ¹ , Nasim Hayati Roodbari ² , Ehsan Ehsani ³ , Azar Sheikholeslami ⁴

1 - PhD student, Department of Biology, Faculty of Convergent Sciences and Technologies, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 - Associate Professor, Department of Biology, Faculty of Convergent Sciences and Technologies, Science and Research Branch, Islamic Azad University, Tehran, Iran
3 - Assistant Professor, Department of Biology, Faculty of Science, Rudhen Branch, Islamic Azad University, Rudhen, Iran
4 - Assistant Professor, Department of Mesenchymal Stem Cells, Academic Jahad, Qom Branch, Qom, Iran.

Received: 2024-08-02 Accepted : 2024-08-23 Published : 2024-08-30

Keywords: endometriosis, resveratrol, stem cells, menstrual blood, women,

Abstract :

Objective: 10% of women of reproductive age experience endometriosis as an inflammatory and hormone-dependent disease. Due to the failure of current treatment approaches, it is necessary to use new and alternative drugs to improve endometriosis treatments. Resveratrol has received much attention due to its many biological effects such as antineoplastic, antioxidant, anti-angiogenic, anti-proliferative, anti-inflammatory and pro-apoptotic properties. The gene expression profile of menstrual blood-derived stem cells (MenSCs) of endometriotic and non-endometriotic women is different. Therefore, in the present study, an attempt was made to evaluate the effects of resveratrol on cell migration and the expression levels of VEGF, KRAS, and IDO1 genes as factors related to cell proliferation in MenSCs of patients with endometriosis.

Materials and methods: MenSCs from healthy women (NE-MenSCs) and endometriosis (E-MenSCs) were isolated and cultured in the laboratory using Faycol solution. E-MenSCs were treated with 100 µM resveratrol for 72 hours, while in
NE-MenSCs and E-MenSCs groups, the cells did not receive any treatment during this period. Investigating the ability of migration and expression level of genes was done using scratch test and real-time PCR.

Findings: After 36 hours, in both NE-MenSCs and Resveratrol groups, the scratch closure percentage was significantly lower than the E-MenSCs group. Also, the expression of KRAS and VEGF genes in the Resveratrol group was significantly lower than the E-MenSCs group.

Conclusion: Resveratrol can reduce the progression of endometriosis by reducing the ability of cells to migrate and reducing the expression of VEGF and KRAS. 

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