Determination of variety of symbiotic bacteria that are isoisolated from alfalfa,s root noudule in yazd province

Subject Areas : cellular , molecular

sohila  sanatizadeh ¹ (student)
Ahmadali  Porbabaee ² (Associate)
Mohammad ali  Amozegar ³ (Associate)

Received: 2014-07-12 Accepted : 2014-08-31 Published : 2014-10-23

Keywords: "Ensifer meliloti", "Rhizobium", "Genotype", noudule, "Phenotype",

Abstract :

Purpose of the study Alfaalfa is a permanent legumes which is widely planted around the world for animal nutrition and is a main source of green fertilizer . This plant is a rare grass that can establish a helpful and nitrogen fixing coexistence with terrestrial – gram negative bacteria of Sinorhizobium .Materials and methodIn this study,after isolated of 50 nodule from alfalfa,s root in fields of Yazd province in spring, 20 bacteria strain that are isolated from nodule were identified regarding their phenotypic traits (microscopic and macroscopic morphology,biological and physiological traits ) and molecular methods using 16SrRNA primer,PCR and sequencing.FindingsRegarding the molecular tests determining its special piece(16SrRNA) and its nearness to so Meliloti , and after determining the sequence Medicago Sativa and 100% similar to Ensifer meliloti, it can be found that Medicago Sativa , which is a bacteria isolated from alfalfa,s , is the same as Sinorhizobium Meliloti . The degree of similarity of three Pseudomonas Koreensis is isolations that had made a noudule was 99% but this doesn,t prove that they also fix the nitrogen. Comparing hemology of sequence of resulting piece of 16SrRNA PCR Sinorhizobium meliloti to 98% has proved that it can be used as a suitable marker of this species in the next experiments. Results Frequency of Ensifer meliloti strains is relatively high in alfalfa in Yazd province and probably the nod genes in Pseudomonas Koreensis isolations cause their ability in nodulation.