Isolation and optimization of keratinase production by Bacillus licheniformis, which produces keratinase enzyme, to break down feathers

Subject Areas : microbiology

Parisa Vanaki ¹ , Fatemeh Zaboli ^{2 *} , Hami Kaboosi ³

1 - Department of Microbiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran
2 - Department of Microbiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran
3 - Department of Microbiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran

Received: 2024-07-11 Accepted : 2024-10-01 Published : 2025-01-12

Keywords: Bacillus licheniformis, Optimization, Keratinase, Animal and Poultry Feed.,

Abstract :

Objective: The Keratinase enzyme produced by Bacillus is used to break down raw keratin and produce animal and poultry feed. The purpose of this study is to investigate the optimization of keratinase-producing bacillus to decompose and provide a protein source for animal feed. Materials and methods: This experimental-laboratory study was conducted in 1401 on chicken slaughterhouse samples. Heat treatment, alcohol treatment, and polymerase chain reaction were used to isolate Bacillus licheniformis (B. licheniformis) strains. To optimally check keratinase activity, keratinase was precipitated by ammonium sulfate method. Comparison of the mean changes and standard deviation of keratinase before and after optimization was done with Tukey's one-way analysis of variance and post hoc test and data analysis was done with SPSS-26 (p<0.05). Findings: (85.57 ± 0.64 units/ml) keratinase activity of B. licheniformis strain PVKR15 was higher than other strains. The total keratinase activity after optimization was 800 units/ml and before optimization was 500 units/ml. pH, temperature, heating time, creatine source, and creatine concentration to optimize conditions respectively; 11, 37 degrees Celsius, 72 hours, and azocreatine at a concentration of 10 g/l. There was a significant difference between the keratinase activity of B. licheniformis PVKR15 with pH, temperature, creatine source, creatine concentration, and incubation time (p<0.05). Conclusion: B. licheniformis PVKR15 has a useful and effective role in the growth of animals to break down keratin as a probiotic supplement for livestock and poultry feed

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