Isolation and identification of extremophile bacteria producing anti-cancer enzyme L-asparaginase from Yale Gonbad hot spring

Subject Areas : biology

Shaghayegh Kashef Haghighat ¹ , Elaheh Ali-Asgari ²

1 - Master's degree, Department of Biology, Tehran East Branch, Islamic Azad University, Tehran, Iran
2 - Assistant Professor, East Tehran Branch, Islamic Azad University, Tehran, Iran

Received: 2024-02-15 Accepted : 2024-04-19 Published : 2024-01-31

Keywords: Extremophile bacteria, L-asparaginase, Anti-cancer enzyme, Yale Gonbad hot spring.,

Abstract :

Purpose: The most abundant sources used to obtain enzymes with greater stability are thermophilic bacteria. In recent years, microbial L-asparaginase has been widely used as a therapeutic agent in the treatment of human cancers. It is also recently used in the treatment of acute lymphoblastic leukemia. In this regard, the aim of this study is to screen new thermophilic strains capable of producing L-asparaginase enzymes.

Materials and methods: Yale Gonbad hot spring was chosen as the isolation site
for thermophilic bacteria. Cultivation and morphological and biochemical tests were
done to identify the strain. The bacterial strain was screened in order to investigate
L-asparaginase enzyme activity by (RPA) method. Modified M9 medium containing
L-asparagine and phenol red was used as reagent. Finally, a bacterial strain was identified by determining the 16RrRNA gene sequence after PCR.

Findings: Finally, a thermophilic strain was isolated from Yale Gonbad hot spring, which belonged to Bacillus licheniformis. L-asparaginase activity was observed in YT strain and Bacillus licheniformis was produced.

Conclusion: Yale Gonbad hot spring is one of the sources with high potential of useful microbial products that can be used as a useful source of many biological products such as enzymes.

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