Evaluation of Resistance of Some Doubled Haploid Lines of wheat to Pathotypes of Yellow Rust Seedling Stages

Subject Areas : crop production

عادله بخشی ¹

1 - دانشجوی دکتری دانشگاه فردوسی مشهد

Received: 2012-02-04 Accepted : 2015-02-19 Published : 2014-12-31

Keywords: doubled haploid lines , resistance , wheat , yellow rust,

Abstract :

Wheat stripe ( yellow ) rust is an important disease in Iran. In order to evaluate the reaction to yellow rust, 64 wheat doubled haploid lines resulted from wheat and maize hybrid chromosome elimination method were tested relative to three wheat yellow rust disease pathogen pathotypes in Sari region. These pathotypes involved 70E34A+, 166E14A+, 70E10A+. Wheat doubled haploid lines were tested in seedling stage in greenhouse condition by three resistant control cultivars ( Parsi, Sivand and Morvarid ) and sensitive Bolani cultivar. All lines and cultivars were planted in three replications in plastic pots in a culture room. In one and two leaves stages, the seedlings were inoculated with urodiniospores of each pathotype separately. After 14 to 25 days, the infected type of each line was recorded based on 0 – 9 scale. Resistance was measured by infected type, latent period, sprout size and sprout density. The analysis of variance showed a significant difference among genotypes. These lines can be used as resistance resources relative to pathotypes in improvement programs. The results indicated that doubled haploid system can be a useful method for production of optimal resistant lines in a short term.

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