Investigation about the Mutagenic and Carcinogenic Effects of Probiotice Bacteria with Using Salmonella typhimurium TA100 and Rat Liver Microsomes(S9)
Subject Areas :maryam EKrami 1 , ُSedigheh mehrabian 2 , Robab Rafiei Tabatabei 3
1 - Msc Department of Microbiology, North Tehran Branch, Islamic Azad University, Tehran. Iran
2 - Professor, Department of Microbiology, Kharazmi University, Tehran. Iran. mehrabians2012@yahoo.com
3 - Associate Professor Department of Microbiology, North Tehran Branch, Islamic Azad University, Tehran. Iran
Keywords:
Abstract :
Inroduction & Objective: Probiotic bacteria is potential hazards should also be considered and used at concentrations that have not mutagenic or carcinogenic effects. This study aimed to assess Investigation about the Mutagenic and Carcinogenic Effects of Probiotice Bacteria with Using Salmonella typhimurium TA100 and Rat Liver Microsomes(S9). Material and Methods:In this study, we utilize the Ames test using Salmonella typhimurium TA100. Firstly, the purity of the strains was confirmed in terms of purity of mutagenic properties. In the next phase of this research the rat liver micrsomes was separately added to the minimal glucose agar medium containing the suspected carcinogenic, andprobiotice bacteria negative and positive controls and all back colonies were counted. Results: The number of revertant colonies the treated plates with S9 is decreased and it means mutagenic and carcinogenic effect of probiotice bacteria with S9 is decreased. Conclusion: The results of the present study shows that the probiotice bacteria at concentrations examined had no mutagenic and carcinogenic effect.
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