Evaluation of serum biochemical profile using black radish (Raphanus sativus) in mice

Subject Areas : Experimental physiology and pathology

Seyedeh Ommolbanin Ghasemian ¹ , Maryam Maryam Karimi-Dehkord ² , Ali Maddahi nejad ³ , Hooman Yousefi ⁴

1 - Department of Veterinary, Behbahan Branch, Islamic Azad University, Behbahan, Iran.
2 - Department of clinical Sciences, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
3 - D.V.M. Student, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
4 - D.V.M. Student, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran

Received: 2023-05-02 Accepted : 2023-08-20 Published : 2024-02-20

Keywords: Black radish, Hydroalcoholic extract, Liver function, Kidney function, Lipid profile,

Abstract :

Background & Aim: Considering the presence of active compounds in black horseradish, the present study aimed to evaluate the effect of hydroalcoholic extract of black radish on liver and kidney function in small laboratory mice. Materials & Methods: In this study, 40 small laboratory mice were randomly divided into four groups of control and experimental groups (50, 100, and 150 mg/ml black radish extract). Intraperitoneal injection of hydroalcoholic extract of black radish was performed in the experimental groups. On the 21st day, the mice were anesthetized and then blood sampling was done. Blood urea nitrogen (BUN), creatinine, alkaline phosphatase (ALP), aspartate aminotransferase (AST), alanine aminotransferase (ALT), triglyceride and cholesterol levels were compared between the groups. Results: The levels of BUN, creatinine, ALP, AST and ALT in the experimental groups were not significantly different from the control group (P>0.05). Treatment with horseradish extract led to a decrease in fat profiles in the experimental group compared to the control group. By increasing the dose of the extract, the amount of triglycerides decreased. This reduction was significant in the highest dose (150 mg/kg) (P<0.05). In addition, black radish extract led to a significant decrease in cholesterol in all three doses compared to the control group (P<0.05). Conclusion: The results of the present study indicate the absence of liver and kidney toxicity of the extract in the doses used in the study. It can also be said that in physiological conditions, the use of black horseradish hydroalcoholic extract reduces the fat profile

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