Induction of obesity, garlic and stevia extract along with aerobic activity, on the expression of semaphorin-4D gene and plexin-B2 receptor in male rats
Subject Areas : Experimental physiology and pathology
Maliheh Ardakanizadeh
1
,
Seyed Javad Ziaolhagh
2
1 - Damghan university, Faculty of sport sciences
2 - Department of Exercise Physiology, Islamic Azad University Shahrood Branch, Shahrood, Iran
Keywords: Obesity, garlic extract, stevia extract, semaphoring-D4, plexin-B2.,
Abstract :
Background and aim: For a long time, garlic fruit and stevia leaves have been expressed as an anti-inflammatory along with aerobic physical activity, on the other hand, semaphorins have recently been identified as proteins that affect central nervous system inflammation. Materials & Methods: the number of 35 obese male Wistar rats (Lee's index) randomly divided into 7 groups (n=5); healthy control, obese, garlic, stevia, aerobic, garlic+aerobic, and stevia+aerobic. Aerobic exercises consisted of 30 M/D, five D/W, and garlic and stevia extract with a concentration of 250 mg/kg was added to the water consumed daily. In order to study the expression of semaphoring-4D gene and its receptor called plexin-B2, brain tissue was sent to the laboratory by real-time PCR method. One-way analysis of variance and Tukey's post hoc test were used to determine the difference between groups (p≤0.05). Results: The expression levels of semaphoring-D4 gene in the obese group increased significantly compared to the healthy control group, and all groups showed a significant decrease in the amount of this gene, except for the aerobic+stevia group. The values of plexin-B2 in the aerobic+stevia group exceeded the values of the obese control group, and the garlic group with/without aerobic activity experienced lower values than the healthy and obese control group (p<0.05). Conclusion: It seems that induction of obesity increases the expression of semaphoring-4D gene and plexin-B2 receptor, and garlic extract and its combination with aerobic activity have beneficial effects on reducing central nervous system inflammation.
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