Effects of Aerobic Exercise and Ethanolic Extract of Purslane Seed on Markers of Oxidative Stress and DNA Damage in lung tissue of Rats Poisoned with Hydrogen Peroxide

Subject Areas :

Shiva  Bahram vash shams ¹ (Department of Exercise Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran)
parvin  farzanegi ² (Associate Professor, Exercise Physiology Department, Sari Branch, Islamic Azad University, Sari, Iran)
Mohammad Ali  Azarbayjani ³ ((PhD) Department of Exercise Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran)

Received: 2022-08-24 Accepted : 2022-12-14 Published : 2022-12-22

Keywords: DNA damage, Aerobic training, Oxidative stress, Purslane Seed, Lung,

Abstract :

Background and aims: The lung is one of the important tissues of the body and is constantly in contact with the highest oxygen pressure and air pollution, which is very sensitive to the production of free radicals due to the low capacity of antioxidant enzymes. The aim of the present study was to evaluateeffects of aerobic exercise and ethanolic extract of purslane seed on ATP, O-6-MethylguanineDNA methyltransferase (MGMT), malondialdehyde (MDA) and prooxidant-antioxidant balance(PAB) levels in the lung tissue of rats poisoned with hydrogen peroxide. Materials & Methods: In this experimental trial, 72 male Wistar rats were randomly divided into nine groups: (1) control + H2O2 , (2) aerobic exercise, (3) aerobic exercise and 50 mg/kg purslane seed extract, (4) aerobic exercise and 200 mg/kg purslane seed extract, (5) aerobic exercise and 400 mg/kg purslane seed extract, (6) 50 mg/kg purslane seed extract, (7) 200 mg/kg purslane seed extract, (8) 400 mg/kg purslane seed extract, and (9) healthy control. Oxidative stress was induced by intraperitoneal injection of 1 mmol/kg hydrogen peroxide three times a week for eight weeks. Aerobic exercise was performed three sessions a week for eight weeks, and the purslane seed extract was intraperitoneally injected daily at the mentioned doses.Results: Aerobic exercise and purslane seed extract alone or combined significantly increased ATP, MGMT and significantly reduced MDA and PAB levels in lung tissue of rats exposed to hydrogen peroxide (P<0.05). Moreover, the effect of purslane seed extract was dose dependent. Conclusion: It seems that aerobic exercise together with consumption of purslane seeds each alone and in combination have interactive effects in reducing oxidative stress, repairing DNA and improving mitochondrial function in the lung tissue of rats poisoned with H2O2.