Comparative study of impact of docetaxel on the cytoskeleton of mouse oocytes after vitrification with two different cryopreservation solutions
Subject Areas :Hamed Daneshpazhouh 1 , Nasim Hayati Roodbari 2 , Mehdi Dianatpour 3 , Zahra Khodabandeh 4 , Yaser Tahamtani 5
1 - Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 - Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
3 - Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
4 - Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
5 - Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran
Keywords: Docetaxel, Vitrification, cryopreservation solution, cryotop, oocytes,
Abstract :
Inroduction & Objective: The aim of the present study was to investigate the effect of docetaxel on the survival rate and in vitro fertilization of oocytes after vitrification by two cryopreservation solution.Materials and Methods: For this NMRI mice (8-10 weeks old) were superovulated by injecting PMSG and HCG. Oocytes are surrounded by cumulus and corona cells and must be denuded by 0.1% hyaluronidase enzyme. The oocytes were then divided into 8 experimental groups including control, docetaxel, docetaxel + vitrification 1 solution; docetaxel + vitrification1; vitrification1; docetaxel + vitrification 2 solution; docetaxel + vitrification2; vitrification2. Mature oocytes were vitrified in ethylene glycol and dimethyl sulfoxide solutions at 15% concentration and 0.5 M sucrose in cryopreservation solution1 and ethylene glycol and glycerol at 7.5 concentration and 0.5 M sucrose in cryopreservation solution2. After thawing, their survival and fertilization rates were assessed up to the two-cell stage. Staining of the microtubules in the oocytes was performed with alpha-tubulin antibody.Results: The results showed a significant difference in survive and fertilization rates compared to the control group (P<0.05). The rate of survival and formation of 2-cell embryos in the first cryopreservation group decreased compared to the second cryopreservation group but the two groups were not statistically significant. The results showed that survival and fertilization rates in pre-incubated groups with docetaxel were higher than non-incubated groups.Conclusion: Docetaxel could improve reproductive techniques by reducing the damage to the oocyte cytoskeleton.
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