The protective effects of hydroalcoholic extract of Passifloracaerulea aerial parts against hepatic dysfunction induced by cadmium chloride in adult male rats

Subject Areas :

Mehrdad Shariati ¹ , Mehrnoush Ghavami ² , Mokhtar Mokhtari ³ , Saeed Khatamsaz ⁴

1 - Department of Biology, Kazerun Branch, Islamic Azad University, Kazerun, Iran
2 - Department of Biology, Kazerun Branch, Islamic Azad University, Kazerun, Iran
3 - Department of Biology, Kazerun Branch, Islamic Azad University, Kazerun, Iran
4 - Department of Biology, Kazerun Branch, Islamic Azad University, Kazerun, Iran

Received: 2020-07-28 Accepted : 2020-11-06 Published : 2020-09-22

Keywords: Adult male rats, Hepatic dysfunction, Passifloracaerulea, cadmium chloride,

Abstract :

Background:Cadmium chloride causes liver injury. In this study, the protective effect of hydroalcoholic extract of Passifloracaerulea aerial parts against hepatic dysfunction induced by cadmium chloride in adult male rats was investigated. Material and Methods:Fifty-four adult male rats were divided into 6 groups of 9:control group; sham group1:received 0.2ml/kg distilled water daily intraperitoneally as solvent. Sham group2;received 2 mg/kg cadmium chloride daily intraperitoneally during 21 days; experimental groups 1, 2 and 3: received 2 mg/kg cadmium chloride daily intraperitoneally during 21 days and then the hydroalcoholic extract of Passifloracaerulea aerial parts at the doses of 150, 300, 450mg/kg intraperitoneally during 30 days . The serum levels of AST, ALT, ALP, GGT, LDH, albumin, bilirubin, and total protein were measured. The pathological examination of hepatic tissue samples were done after hematoxylin - eosin staining. Results:The mean levels of ALT, ALP, GGT, LDH and bilirubin in the all experimental groups showed a significant decrease compared to the group receiving cadmium chloride. The mean levels of AST in the experimental groups 2 and 3 showed a significant decrease compared to the group receiving cadmium chloride. The mean serum albumin and total protein concentration in experimental groups 2and 3 increased significantly compared to the group receiving cadmium chloride. In all experimental groups the hepatic tissue changes induced by cadmium chloride improved which were dose dependent (P˂0.05). Conclusion:The results of this study showed that the hydroalcoholic extract of Passifloracaerulea aerial parts had protective effects against the hepatic dysfunction induced by cadmium chloride in adult male rats.

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