Comparison of the Effect of Hydroalcoholic Extract of Licorice (Glycyrrhiza glabra) and Its Active Ingredient Glycyrrhizic Acid on the Amount of Bax and Bcl-2 Proteins in the Liver Cells of Type 1 Diabetic Male Rats

Subject Areas :

Sedigheh Jani ¹ , Vida Hojati ² , Gholamhassan Vaezi ³ , Raheleh Rahbarian ⁴

1 - Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran
2 - Department of Biology Damghan Branch, Islamic Azad University, Damghan, Iran
3 - Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran
4 - Department of Biology, Payame Noor University, Tehran, Iran

Received: 2021-08-23 Accepted : 2021-10-07 Published : 2021-09-23

Keywords: Alloxan, licorice, BAX, BCL2, Rat,

Abstract :

Introduction and Objective: The function of the liver as an insulin-dependent organ is strongly affected by diabetes. Diabetes can lead to liver damage through oxidative stress. The present study was performed to evaluate the possible effects of hydroalcoholic extract of licorice (Glycyrrhiza glabra) and glyceric acid on Bax and Bcl2 apoptotic factors in male type 1 diabetic rats. Material and Methods: In this experimental study, 72 male Wistar rats were injected into diabetic control, diabetic control and diabetic groups treated with hydroalcoholic extract of licorice and glycyrrhizinic acid at concentrations of 50 and 100 mg / kg by injection. The time intervals of 15 and 30 days were divided. Diabetic control and treatment groups became diabetic with a single intraperitoneal injection of 120 mg/kg alloxane monohydrate. At the end of the treatment period, Bax and Bcl2 apoptotic factors were measured. The results were analyzed by SPSS 21 software and one-way analysis of variance and Tukey post hoc test. Results: Bax apoptotic factor was significantly decreased in the treated groups compared to the diabetic control groups and Bcl2 anti-apoptotic factor was significantly increased (p <0.05) which indicates improvement in the structure of damaged liver tissue in the groups. It is a treatment. Conclusion: Hydroalcoholic extract of licorice and glycyrrhizic acid is in expressed doses of anti-apoptotic and has a protective effect against liver damage induced by diabetes.

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