Effect of Copper Sulfateon the Wistar Rat's Fetal Liver Tissue
Subject Areas :Rahil Jannatifar 1 , Ahmad Farrokhi 2 , Sona Zargari 3 , Mehrdad Ghorbanlou 4 , Reza Nejatbakhsh 5
1 - مرکز درمان ناباروری جهاد دانشگاهی، قم. ایران.
2 - گروه علوم تشریح، دانشکده پزشکی، دانشگاه علوم پزشکی زنجان. ایران
3 - گروه علوم دامی، دانشکده کشاورزی، دانشگاه زنجان. ایران.
4 - گروه علوم تشریح، دانشکده پزشکی، دانشگاه علوم پزشکی زنجان. ایران
5 - گروه علوم تشریح، دانشکده پزشکی، دانشگاه علوم پزشکی زنجان. ایران
Keywords: liver, fetus, Keywords: Copper Sulfate, Rat,
Abstract :
Inroduction & Objective: Heavy metals like copper, even at low concentrations, can damage the tissue of creatures. According to high doses of copper in neonate's liver and the importance of liver development, this study was conducted by the purpose of the evaluation of the effect of copper sulfate on the Wistar rat's fetal liver tissue.Material and MethodIn this experimental study, 48 male and female Wistar rats with weight range of 200 to 250 gr were used. Before pregnancy, adult female rats were divided randomly into four control and copper sulfate experimental groups. Experimental groups respectively delivered 0.25, 0.5 and 1 gr copper sulfate in each 1000 gr of standard diet for 2 weeks before mating and during pregnancy. At the 21th day of pregnancy, rats were euthanized and the fetuses were removed from the female uteri and the liver was prepared for the purpose of histological studies. Data were analyzed by SPSS software and P was considered significant.Results: Average cephalocaudal length in the control group in comparison to experimental groups of 1 and 3 indicated significant difference (PConclusions:Copper sulfate at average and higher concentrations may have destructive effects on histology and cells of fetal liver. These changes and damages can be a function of the amount of consumption dose, time period and consumption period of copper sulfate