Study of Changes Histomorphometry Spleen in Rats with Pregnancy and Lactation
Subject Areas :elham Etemadi 1 , Akbar Karimi 2 , Ali Asghar Pilevarian 3
1 - کارشناسی ارشدگروه زیست شناسی، دانشگاه پیام نورتهران، تهران. ایران.
2 - استادیار گروه زیست شناسی، دانشگاه پیام نور تهران، تهران. ایران
3 - دانشیار گروه زیست شناسی، دانشگاه پیام نور تهران، تهران. ایران.
Keywords: Lactation, spleen, Keywords:Pregnancy, Histomorphometry,
Abstract :
Inroduction & Objective: Major changes occurred in the mother's body during pregnancy, sometimes these changes for both mother and fetus at risk by recognizing the changes in the conditions of a woman's pregnancy can be managed. In this study, histomorphometry changes of spleen tissue during pregnancy and lactation in female Wistar rats were studied.Materials and Methods:In this study, 54 Wistar rats (12 male and 42 female) with an average weight of 220-240 g and after breeding and observation of vaginal plug 18 pregnant rats into 3 groups of 6 rats and 6 Rat non-pregnant adult female as control groups, and including the first day of pregnancy into 4 groups, control, 10 and 20 of pregnancy and the first week of lactation were divided. Then the rats were dissected and spleen tissue for histopathological examination with hematoxylin-eosin out and were. In cases the number of macrophages, white pulp megakaryocytes and diameter were evaluated. Statistical analysis ANOVA and Duncan test were examined. Results:The results indicated that in spleen tissue number of macrophages at 10 and 20 and diameter(P ConclusionProcesses of pregnancy increases changes done in spleen tissue included increases white pulp and macrophages. These changes are dependent on mother's immune system