Study Of the Hepatoprotective Effect Of Capparis spinosa Fruit Extract against Acetaminophen-Induced Toxicity in Rat
Subject Areas :Masoumeh Eslami 1 , ّّFarah Farrokhi 2
1 - کارشناس ارشد فیزیولوژی، گروه زیستشناسی، دانشکده علومپایه، دانشگاه ارومیه، ارومیه. ایران
2 - دانشیار بافتشناسی، گروه زیستشناسی، دانشکده علومپایه، دانشگاه ارومیه، ارومیه، ایران.
Keywords: Hepatotoxicity, Acetaminophen, Capparis Spinosa,
Abstract :
Introduction and Objectiv:Acetaminophen(paracetamol, APAP) is a widely used analgesic and antipyretic drug. At the therapeutic doses, APAP is considered a safe drug. However, its toxic doses can lead to liver damage and is often fatal. The hepatotoxicity of APAP is mainly a result of oxidative stress mediated by the metabolite of APAP, that is, N-acetyl-para-benzoquinon imine (NAPQI).The present study was undertaken to evaluate protective activity of the aqueous extract of Capparis spinosa(Capparidaceae) fruit against APAP-induced hepatotoxicity in rats.Material andMethods:In this study, animals randomly were divided into five groups (6 rats in each group). The safe and APAP-intoxicated control groups were received normal saline and the remaining groups received aqueous extract with different doses 50, 100 and 200 mg/kg by gavage. For induction of hepatotoxicity, in the end of the treatment, APAP (500 mg/kg, orally) were given animals except safe control group.Results:The serum levels of alanine aminotransferase(ALT), aspartate aminotransferase(AST), alkaline phosphatase(ALP), and total bilirubin significantly decreased in the groups received the extract when were compared to APAP-intoxicated control (PConclusion:These findings suggest that C. spinosa fruit extract can prevent APAP-induced hepatotoxicity in rats and this is likely mediated through its antioxidant properties