Effect of Green Tea Extract on HER2 Gene Expression in Gastric Cancerous Cells Line (AGS)
Subject Areas :Sepiedeh Derakhshan 1 , Zahra Deilami Khiabani 2
1 - کارشناس ارشد ژنتیک، گروه ژنتیک، دانشکده علوم پایه، دانشگاه آزاد اسلامی واحد زنجان، زنجان.ایران.
2 - استادیار،گروه ژنتیک، دانشکده علوم پایه، دانشگاه آزاد اسلامی واحد زنجان، زنجان. ایران
Keywords: HER2, gastric cancer, Green tea,
Abstract :
Inroduction & Objective: Gastric cancer is second leading cause of cancer related death in the world. Use of anticancer herbal drugs because of having fewer side effects is of importance. green tea is one of herbal medicines which contain anticancer effect.The aim of this study has been to investigating changes in gene expression of HER-2 in green tea treated human AGS adenocarcinoma cells lines. Material and Methods:AGS cells were treated with the aqueous extract of green tea in different concentrations including 800,1200 and 2000 μg/ml. After 48 hrs and 72hrs treatment, RNA extraction has been done. Following cDNA synthesis, the rate of expression of HER-2 gene was evaluated using Real time PCR. Results: The over expression of HER-2 gene has been shown in 800 μg/ml of green tea extract. In dose of 1200 μg/ml and 2000 μg/ml of green tea extract significant reduction in HER-2 gene have been shown and the most reduction has been observed in 72 hours treatment and the concentration of 2000 μg/ml green tea extract.Conclusion:Green tea extract in high concentrations, is the reason of decreasing the rate of HER2 gene expression and this factor can prevent the development and progression of gastric cancer cells.