The Synergic Effects Of Shell Extract Of Persian Gulf ChitonLamyiand Acellular Brain on Angiogenesis in Chick Embryo Chorioallantoic Membrane
Subject Areas :جواد بهارآرا 1 , تکتم جوان جعفری بجنوردی 2 , ناصر مهدوی شهری 3 , سعیده ظفربالا نژاد 4
1 - دانشیار، دکتری تخصصی زیست شناسی تکوین جانوری، گروه زیست شناسی و مرکز تحقیقات بیولوژی کاربردی تکوین جانوری دانشگاه آزاد اسلامی مشهد، دانشگاه آزاد اسلامی واحد مشهد، مشهد. ایران
2 - دانشجوی کارشناسی ارشد زیست شناسی سلولی تکوینی، گروه زیست شناسی دانشگاه آزاد اسلامی واحد مشهد، مشهد. ایران.
3 - استاد، دکتری تخصصی هیستولوژی، گروه زیست شناسی، دانشگاه فردوسی مشهد. ایران.
4 - مربی، گروه زیست شناسی، دانشگاه آزاد اسلامی واحد مشهد، مشهد. ایران.
Keywords: Rat, acellular brain, Chiton, Chorioallantoic Membrane, angiogenesis,
Abstract :
Inroduction and Objective: Chitons are important for medical research because exist of chitosan and chitin in itʼs shell. Because of importance of angiogenesis and since Angiogenesis is a complex process involving extensive interplay between cell soluble factors and extra cellular matrix (ECM) components. In this study, we investigated the angiogenic response induced by Synergic effects of chitonʼs shell extract and acellular brain scaffolds implanted onto the chick chorioallantoic membrane.Materials and Methods In this experimental study, the rat brain was decellularized. we used 50 fertilized eggs that were divided into 5 groups: control, sham-exposed1, sham-exposed2, experimental 1,and experimental 2. In the 12nd day of incubation, the numbers and lengths of vessels around the sponges were measured by Image J software, data were analyzed with SPSS (version 16) and by statistic test such as ANOVA and Tukey results in significant level PResults: Mean number(10/34 ± 1/85) and length(13/12±2/04mm) of vessels in the control with number(6/06±1/36) and length(9/76±1/21mm) of vessels in the experimental group 1 showed a significant decrease (p Conclusion:Results indicate that synergic usage of shell extract of Chiton Lamyi and acellular brain decrease angiogenesis in chorioallantoic membrane