Number 3 Volume 1

Open Access Article

1 - Recombinant lactococcus, a new approach for oral vaccines
علی شریف زاده sara arti fatemeh shayesteh

Recombinant probiotics are increasingly acting as carriers for the expression and targeted delivery of recombinant or natural molecules to mucosal surfaces in nutrition and health. Probiotics-mediated delivery systems eliminate the need to purify molecules to a large ex More

Recombinant probiotics are increasingly acting as carriers for the expression and targeted delivery of recombinant or natural molecules to mucosal surfaces in nutrition and health. Probiotics-mediated delivery systems eliminate the need to purify molecules to a large extent and make it possible to deliver molecules to the mucosa. The concept of biological medicine is the oral administration of living recombinant microorganisms for the prevention or treatment of various diseases. Lactococcus lactis is a gram-positive bacterium from the group of lactic acid bacteria. Lactococcus lactis has been used for centuries in food fermentation, especially cheese, yogurt, sauerkraut and the like. Therefore, it is generally recognized as a safe situation by the Food and Drug Organization. In this review article, review articles related to lactococcus and its wide application in oral vaccine production from 1998 to 2020 were collected. Accordingly, the following databases were searched. Scopus, PubMed and Google Scholar databases. The keywords used in this study included "immunity, Lactococcus lactis and vaccine". Lactococcus lactis is generally recognized as safe and can be widely used in the food industry. Live recombinant Lactococcus lactis, as a biological drug, is administered orally as a live vaccine that expresses viral and bacterial antigens. Manuscript profile

Open Access Article

2 - Investigation of contamination of Vibrio species in fish sold in the market of Shahrekord city
مهران خلیلی دهکردی Fereshteh Salehian dehkordi Majid Esmaeili vardanjani

Today, consumption of seafood has a special place among consumers. Consumption of seafood can lead to the transmission of bacterial pathogens of primary or secondary origin, among the most important of these bacteria are Vibrio species that are naturally found in aquati More

Today, consumption of seafood has a special place among consumers. Consumption of seafood can lead to the transmission of bacterial pathogens of primary or secondary origin, among the most important of these bacteria are Vibrio species that are naturally found in aquatic environments and in the bodies of aquatic organisms. Every year, many cases of food poisoning caused by Vibrio species are reported in consumed fish. The purpose of this research is to investigate the level of Vibrio contamination of raw fish sold in Shahrekord city. In this research, 50 fish samples were prepared from 30 fish and aquatic supply stores in Shahrekord city and then analyzed at the Nutrition and Organic Products Research Center of Islamic Azad University, Shahrekord branch. Vibrio parahaemolyticus, Vibrio cholerae, Vibrio vulnificus, Vibrio mimicus and Vibrio alginolyticus can be mentioned among the most important species investigated. Then, based on the presence or absence of grown spores, 40% of the meat of the studied samples (20 pieces) were infected with Vibrio. The high abundance of Vibrio species in the samples confirms the lack of hygiene in the fish and its products preparation and distribution centers. It seems that the processing stations and the method of transporting and distributing fish do not have proper hygiene. Manuscript profile

Open Access Article

3 - Investigation of Salmonella enteritidis contamination in chicken and quail meat sold in Khuzestan province
Zahra Motaghi Hossein Khodabandeh shahraki mahla elahinia

Salmonellosis is caused by Salmonella bacteria and is one of the most common food-borne diseases that is accompanied by diarrhea, mild fever, nausea, abdominal pain and even death. This study was conducted in order to isolate and identify common Salmonella serotypes in More

Salmonellosis is caused by Salmonella bacteria and is one of the most common food-borne diseases that is accompanied by diarrhea, mild fever, nausea, abdominal pain and even death. This study was conducted in order to isolate and identify common Salmonella serotypes in chicken and quail meat. For this purpose, 100 meat samples, including 50 chicken meat and 50 quail meat samples, were collected from distribution centers in Khuzestan province and after cultivation and isolation of salmonella and genome extraction, they were analyzed by polymerase chain reaction method. The results showed that 5 out of 100 samples were infected with Salmonella enteritidis. According to the results, it is very important to apply periodic microbial monitoring and tests and more measures from the officials of the provincial veterinary department in order to reduce the amount of microbial contamination. Manuscript profile

Open Access Article

4 - Prevalence of sul genes in E.coli isolated from urinary tract infectious in Shahrekord
Mahnaz Shamaei Maryam Reisi Hossein Khodabandeh shahraki

Urinary tract infections is one of the most common infectious diseases and E. coli is one of urinary tract infection the most important factor. The purpose of this investigation is prevalence of sul genes in E.coli isolated from urinary tract infectious in Shahrekord to More

Urinary tract infections is one of the most common infectious diseases and E. coli is one of urinary tract infection the most important factor. The purpose of this investigation is prevalence of sul genes in E.coli isolated from urinary tract infectious in Shahrekord to form cross-sectional in 2013. Samples was prepared as sterile and in terms of urine tests, cultures and was studied. Investigation antimicrobial susceptibility was performed by disk diffusion method. As well as, for tracing Sul gene PCR reaction was performed in the presence of specific primers and the results was analyzed. In this study of 130 E. coli isolates studied 67 isolates (53/51%) resistance to co-trimoxazol was observed. The frequency of genes sul1, sul2 and sul3 was respectively 20/89%, 55/22% and 4/47%. In statistical analysis with chi-square test between to resistance sulfonamides and sul genes significant correlation was observed. The results showed that E. coli isolates are high resistant to sulfonamides that may be the indiscriminate use of these antibiotics. Manuscript profile

Open Access Article

5 - Investigate the frequency of genes sul and investigate the relationship between genes sul and Class I integrons in Klebsiella pneumoniae isolates resistant to sulfonamides isolated from clinical cases in Shahrekord
Marziyeh Farsinejad Maryam Reisi JAMSHID Alibabaeishahraki Hossein Khodabandeh shahraki

Antimicrobial resistance is a common problem throughout the world. Acquire integrons is one One of the main causes multi- resistance in gram-negative microorganisms. The purpose of this study, Investigate the frequency of genes sul and investigate the relationship betw More

Antimicrobial resistance is a common problem throughout the world. Acquire integrons is one One of the main causes multi- resistance in gram-negative microorganisms. The purpose of this study, Investigate the frequency of genes sul and investigate the relationship between genes sul and Class I integrons in Klebsiella pneumoniae isolates resistant to sulfonamides isolated from clinical cases in Shahrekord. In this study, antibiotic resistance 90 isolates of Klebsiella pneumoniae isolated from clinical cases of Shahrekord,disk diffusion method was performed. In order to investigate resistance to sulfonamides of the antibiotic cotrimoxazole was used. Then using specific primers was performed tracing genes sul1, sul2 and sul3 and intI. After PCR reaction of 33 isolates resistant to trimethoprim-sulfamethoxazole sul1 gene in 15 isolates (45/45%), sul2 gene in 20 isolates (60/60%), gene Sul3 in 2 isolates (6/06%) and the gene intI 27 isolates was found. The statistical analysis between genes sul1and IntI significant relationship was observed. The results of this study show that is a strong correlation between carry integrons and increased resistance to a number of different classes of antibiotics. Manuscript profile

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