Determination of phenotypic and genotypic pattern of antibiotic resistance in Escherichia coli isolates isolated from diabetic patients in Shahrekord
Subject Areas : microbiologyfatemeh Khodaverdipour 1 , Amin Roozbehi 2
1 - Department of Microbiology, Faculty of Basic Siences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
2 - Department of Microbiology, Faculty of Basic Siences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
Keywords: Antibiotic resistance, Diabetic patients, Uropathogenic E. coli,
Abstract :
Introduction: Preventing the spread of drug resistance is one of the most important issues in society. Escherichia coli is one of the most common bacterial agents isolated from the urinary tract and nosocomial infections. Treatment of infections due to it is difficult due to the acquisition of resistance genes. This study aimed to investigate the phenotypic and genotypic pattern of antibiotic resistance in clinical uropathogenic strains isolated from diabetic patients. Materials and methods: A total of 51 E. coli isolates from urinary tract infection in diabetic patients obtained from clinical, were used in this study. Isolates were confirmed by chemical tests and molecular techniques based on tracking of the 16srRNA gene. Antimicrobial resistance assessment of isolates was done using molecular methods base on (qnrA, tet A, tet B, aac (3)IIa, sul1) and disk diffusion. Results: Most resistance r to ampicillin (66.66%) and the lowest resistance to Nitrofurantoin (1.96%) were reported. The frequency of tet A, tet B, qnr A, sul 1 and aac (3) IIa genes reported 68.62%, 64.7%, 29.41%, 39.21%, and 29.41% respectively. The statistical analysis shows a significant relationship between resistance to the antibiotic tetracycline and the gene tet A, tet B statistically. Conclusion: Early detection of resistant strains to select the most appropriate treatment options is essential to prevent the spread of resistance. It is suggested, as treatment for urinary tract infections is important, so to prevent drug resistance and treatment failure, it should be done according to the resistance pattern in the region.
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