Molecular analysis of Hepatitis delta virus
Subject Areas : Molecular genetics of microorganismsElahe Tajbakhsh 1 * , fatemeh Khodaverdipour 2
1 - گروه زیست شناسی دانشگاه آزاد اسلامی واحد شهرکرد
2 - Department of Biology, ShK.C., Islamic Azad University, ShahreKord, Iran.
Keywords: genotype II HDV, Hepatitis Delta Virus, Similarity genetic,
Abstract :
Hepatitis delta virus (HDV) is an RNA virus that can lead to severe acute, and chronic forms of liver disease using the helper function of the hepatitis B virus. HDV strains are categorized into three genotypes and eight clades, which distribute geographically. The prevalence rate of HDV infection varies from 2.4 to 10 percent in blood donors for chronic liver disease in Iran. The aim was to find out the phylogenetic background of samples isolated in Shahrekord, Iran.
A total of 300 HBsAg positive sera samples that were found blood donors and HBsAg positive patients’ refer to blood transfusion center and clinical laboratory in Shahrekord, Iran. HDV RNA was extracted using RNXPlus (CinnaGen, Iran). 421 bp corresponding to HD Ag have been isolated from Hepataitis Delta Virus in Shahrekord, then were amplified in PCR system And 412 bp fragments for sequencing PCR products were sent to participate macro genes Korea.
Total of 350 serum samples studied in two cases (0/57%) HDV infection was observed in the RT-PCR method. HDVAg Iranian isolates sequenced in this study were compared with 15 sequences from isolates HDV from different countries. 2 positive samples under study were compared by clustral X software with registered sequences of this gene in NCBI gene bank. After specifying the amount of similarity, obtained phylogenic tree was drawn by Njplot software. Sequence of HDV isolates from different countries. Samples under study had 99/1-99/7 percent similarity to sequences of this virus from southeastern Asian countries (china and Taiwan) and based on previous studies, we can put these viruses in genotype II branch of HDV virus.
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