Characterization of functionally graded hydroxyapatite/TiO2 multilayer coating on Ti-6Al-4V titanium alloy by electrophoretic deposition

Subject Areas :

محمد جعفر  هادیان ¹ (استاد)
علیرضا  عراقی ² (استاد)
طاهره  طلایی ³ (استاد)
مهسا  ثانی ⁴ (مربی)

Received: 2015-10-03 Accepted : 2016-09-13 Published : 2016-08-22

Keywords: Electrophoretic, hydroxyapatite, Cell Culture, Functionally graded,

Abstract :

Single layer and functionally graded coatings of hydroxyapatite and TiO2 particles were deposited on Ti-6Al-4V titanium alloy substrate by electrophoretic deposition technique (EPD). The coatings chemical composition and morphology were investigated using energy dispersive X-ray spectroscopy (EDX), scanning electron microscope (SEM) and X-ray diffraction (XRD) methods. The results showed in HA/TiO2 functionally graded coating, the composition varied from inner layer to top layer as 100 %wt TiO2 to 100 %wt HA. To investigation biocompatibility of the coatings, the culture Wharton’s Mesenchymal Stem Cells was used. The results showed the HA/TiO2 functionally graded coating and HA single layer coating were more biocompatible in comparison to TiO2 single layer coating. The adhesion strengths of the coatings were measured by shear testing and the results showed that the HA/TiO2 functionally graded coating has more adhesion strength (~31 MPa) compare to HA single layer.

References:

[1]     L. Mohan, D. Durgalakshmi, M. Geetha, T. S. N. Sankara Narayanan & R. Asokaman, “Electrophoretic deposition of nanocomposite (HAp + TiO₂) on titanium alloy for biomedical applications”, Ceramics International, Vol. 38, pp. 3435-3443, 2012.

 

[2]     P. C. Rath, L. Besra, B. P. Singh & S. Bhattacharjee, “Titania/hydroxyapatite bi-layer coating on Ti metal by electrophoretic deposition: Characterization and corrosion studies”, Ceramics International, Vol. 38, pp. 3209-3216, 2012.

 

[3]     O. Albayrak, O. El-Atwani & S. Altintas, “Hydroxyapatite coating on titanium substrate by electrophoretic deposition method: Effects of titanium dioxide inner layer on adhesion strength and hydroxyapatite decomposition”, Surface & Coatings Technology, Vol. 202, pp. 2482-2487, 2008.

 

[4]     C. Kaya, “Electrophoretic deposition of carbon nanotube-reinforced hydroxyapatite bioactive layers on Ti-6Al-4V alloys for biomedical applications”, Ceramics International, Vol. 34, pp. 1843-1847, 2008.

 

[5]     X. Pang & I. Zhitomirsky, “Electrophoretic deposition of composite hydroxyapatite–chitosan coatings”, Materals Characterization, Vol. 58, pp. 339-348, 2007,

 

[6]     M. Manson, C. J. Nez, C. Morant, P. Herrero & J. M. M. H. Nez-Duart, “Electrodeposition of hydroxyapatite coatings in basic conditions”, Biomaterials, Vol. 21, pp. 1755-1761, 2000.

 

[7]     L. L. Hench, “Bioceramics: from concept to clinic”, Journal of American Ceramics Society, Vol. 74, pp. 1485-1510, 1991.

 

[8]     R. Z. Legeros, “Biodegradation and bioresorption of calcium phosphate ceramics”, Clinical Materials, Vol. 14, pp. 65-88, 1993.

 

[9]     C. F. Koch, S. Johnson, D. Kumar, M. Jelinek, D. B. Chrisey, A. Doraiswamy, C. Jin, R. J. Narayan & I. N. Mihailescu, “Pulsed laser deposition of thin films”, Materials Science Engineering, Vol. 27C, pp. 484-494, 2007.

 

[10] S. Ding, “Properties and immersion behavior of magnetron-sputtered multilayered hydroxyapatite /titanium composite coatings”, Biomaterials, Vol. 24, pp. 4233-4238, 2003.

 

[11] W. R. Lacefield, L. L. Hench & J. Wilson (Eds.), “An Introduction to Bioceramics”, World Scientific Publishing Co, Singapore, 1993.

 

[12] Araghi & M. J. Hadianfard, “Fabrication and characterization of functionally graded hydroxyapatite/TiO2 multilayer coating on Ti-6Al-4V titanium alloy for biomedical applications”, Ceramics International, Vol. 41, pp. 12668–12679, 2015.

 

[13] T. S. Karande, J. L. Ong & C. M. Agrawal, “Diffusion in musculoskeletal tissue engineering scaffolds: design issues related to porosity, permeability, architecture and nutrient mixing”, Annals of Biomedical Engineering, Vol. 32, pp. 1728-1743, 2004.

 

[14] S. Sosnowski, P. Wozniak & M. Lewandowska-Szumiel, “Polyester scaffolds with bimodal pore size distribution of tissue engineering”, Macromolecular Bioscience, Vol. 6, pp. 425-434, 2006.

[15] T. R. Mosmann, “Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assay”, Journal of Immunological Methods, Vol. 65, pp. 55-63, 1983.