Effect of foliar application of putrescine on antioxidative defense of wheat (Triticum aestivum L. var sw _82_9) under water deficit stress

Subject Areas :

Zahra Karimi ¹ (M.Sc. of Agronomy, Department of Agriculture, Islamic Azad University, Varamin Branch, Varamin, Iran)
Hamid Reza Tohidi Moghadam ² (Assistant Professor of Agronomy, Department of Agriculture, Islamic Azad University, Varamin Branch, Varamin, Iran)
Pourang Kasraei ³ (Assistant Professor of Agronomy, Department of Agriculture, Islamic Azad University, Varamin Branch, Varamin, Iran)

Received: 2015-07-09 Accepted : 2015-12-05 Published : 2014-11-22

Keywords: Wheat, Water deficit stress, putrescine, Antioxidative enzymes,

Abstract :

This study was carried out to assess effect of foliar application of putrescine on biochemical enzymes of wheat (Triticum aestivum L .var sw _82_9) under water deficit stress as split factorial experiment with complete randomized  block design ,four treatment and three replication of putrescine on a farm at Islamic Azad University, Varamin branch at 2014. main plots were including four irrigation treatments (Normal irrigation, irrigation withholding at stem elongation stage, irrigation with holding at flowering stage and irrigation withholding at seed filling period stage) and in sub plot units putrescine was used at three concentrations (0, 150 and 300ppm).Results showed that the highest SOD and the lowest SOD and CAT activities belonged to irrigation withholding at seed filling period stage and normal irrigation ,respectively(P < 0.05). The highest and lowest MDA activity belonged to foliar application of putrescine at 0 and 150 ppm, respectively. Putrescine foliar application decreased proline malondialdehyde and dityrosine in leaves by reduction of water deficit stress. In addition, plants were preferred putrescine utilize as a scavenger than the enhancement enzyme activity in against stress and perhaps putrescine foliar application with 150ppm concentration could indirectly decrease oxidative stress.