Assessment of the different treatments effect on seed dormancy break in Ferula gummosa Boiss

Subject Areas :

Soudabeh  Ahooyi ¹ (MSc graduated of seed science and technology, College of Agriculture, Islamic Azad University of Mashhad Branch, Mashhad- Iran.)
Alireza  Sohani Darban ² (Agronomy and plant breeding department, College of Agriculture, Islamic Azad University of Mashhad Branch, Mashhad- Iran.)
Mohsen  Nabavi Kalat ³ (Agronomy and plant breeding department, College of Agriculture, Islamic Azad University of Mashhad Branch, Mashhad- Iran.)

Received: 2016-07-11 Accepted : 2016-12-22 Published : 2016-08-22

Keywords: germination, Gibberellic acid, Cold stratification, KNO3,

Abstract :

Ferula gummosa owned umbelliferus of medicinal plants, industrial and pasture is important. One of the major problems in the field of these domestication species is the presence of seed dormancy. Therefore, it seems absolutely necessary to evaluation of break seed dormancy in this plant. Aim of this study is dormancy breaking, germination and evaluating the best treatment for the elimination of Ferula gummosa seed dormancy. For this purpose, an experiment was carried out as factorial in based on completely randomized design with three replications in 1392, that it was conducted in the laboratory of Islamic Azad University of Mashhad. To perform the experiment, Ferula gummosa seed were collected from Binalud Highlands city of Nishabur in August 1391. The experiments included cold stratification at four levels (30, 45, 60 and 70 days) with treatment gibberellic acid at four levels (control, 250 ppm, 500 ppm and 1000 ppm) and also cold stratification at four levels (30, 45, 60 and 70 days) with treatment kno3 at four levels (control, 1%, 2% and 3%). The final assessment of germination, after 3 weeks of maintenance conducted on Germinator. The results of the first experiment showed that cold stratification and gibberellic acid had a significant positive effect on germination of Ferula gummosa and the maximum percentage (67.5 %) and rate of germination (2.45 seed/day) were obtained by treatment of 60 days stratification. The lowest percentage and rate of germination was gained by treatment of 30 days stratification. Application of 250 ppm gibberellic acid had the highest germination rate (1.94 seed/day) among different levels of gibberellic acid. The results of the second experiment illustrated that cold stratification and kno3 caused to improve germination properties and the highest level of kno3 caused to gain the highest percentage (51.66 %) and rate of germination (1.31 seed/day). No-application of kno3 had the lowest amount of these properties (percentage and rate germination were 31.66% and 0.68 seed/day, respectively).