The study effectiveness of Satureja khuzestanica extract on Leishmania major in vitro condition

Subject Areas : Large Animal Diseases

M. Asmar ¹ , S. Asiabi ² , R. A. Fattahi ³ , لیلا Modiri ⁴ , مهین Farahmand ⁵ , Kh Issazade ⁶ , Sh. Yavari ⁷

1 - pasteur institute Tehran, iran
2 - Msc microbiology Islamic azad university of lahijan
3 - faculty of Islamic azad university of lahijan
4 - Msc veterinary parasitology university of tehran
5 - pasteur institute Tehran, iran
6 - Msc veterinary parasitology university of tehran
7 - faculty of Islamic azad university of lahijan

Received: 2012-05-24 Accepted : 2012-06-22 Published : 2015-02-20

Keywords: Leishmania major, Glucantime, Satureja khuzestanica extract,

Abstract :

This study aimed to determine effectiveness of Satureja khuzestanica extract on Leishmania major in vitro. In present study standard strain of Leishmania major with MRHO/IR/75/ER international code was used that previously had been cultured in NNN medium. This parasite was transferred from NNN medium to RPMI 1640 medium. Then at logarithmic phase, certain numbers of parasite were taken from this medium and were added to cell culturing plate. Then effect of 250 , 500 , 1000 mg /ml extract of Satureja khuzestanica on Leishmania major promastigotes were examined in 24 and 72 hours and the effect of these concentrations were compared to glucantime. Additionally, effects of concentrations such as 250, 300, 350, 400, 450 mg / ml of Satureja khuzestanica extract was examined to obtain its minimum effective concentration. In the study and comparison between extract and glucantime with concentrations 250 , 500 , 1000 , mg /ml , after 72 hours , decrease in promastigote population were compared to untreated control in these concentrations and for Satureisa khuzestanica extract were 56.14% , 77.19% and 77.19% , respectively while for glucantime it was 19.29 , 24.56 , 33.33% respectively. Minimum effective concentration of this extract was up to 250 mg /ml . Satureja khuzestanica extract has an acceptable effect on Leishmania major in in vitro conditions in comparison with glucantime

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