Introduction of ELISA based on MBP-G1 protein for detection of bovine ephemeral fever virus-infected water buffalo
Subject Areas : Large Animal Diseases
Keywords: Escherichia coli, Elisa, water buffalo, recombinant MBP-G1 protein, Bovine ephemeral fever virus (BEFV),
Abstract :
Bovine ephemeral fever (BEF) is a non contagious disease of cattle and water buffalo, spanning in tropical and subtropical zones such as Asia, Australia, and Africa. The aim of this study was to investigate the reactivity of the fusion MBP-G1 protein expressed in Escherichia coli in an indirect in-house ELISA. In order to, after cloning of the G1 gene of bovine ephemeral fever virus (BEFV) G glycoprotein into pMalc2x vector and subsequently expression in Rosetta strain of Escherichia coli, recombinant MBP-G1 proteinwasused as a coating antigen to develop an ELISA. Results showed that the expressed G1 protein could react with immunized mice serum with commercial BEF vaccine and detect of antibodies against BEFV. Thus, the expressed fusion MBP-G1 protein can be introduced as an ideal candidate to develop an ELISA for detection of BEFV-infected water buffalo.
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