Genotyping of BVDV type 1 and 2 isolated from PI cattle in Tehran province by multiplex PCR

Subject Areas : clinical veterinary science

فرهاد Moosakhani ¹ , آریا Badiei ² , مهدی Loghmani ³ , علیرضا Shaghayegh ⁴ , محسن Zafari ⁵

1 - Department of Pathobiology, Faculty of Veterinary Medicine, Islamic Azad University, Karaj branch, Karaj, IRAN
2 - Department of Clinical Sciences, Faculty of Veterinary Medicine, Islamic Azad University, Karaj branch, Karaj, IRAN
3 - Graduated from Faculty of Veterinary Medicine, Islamic Azad University, Karaj branch, Karaj, IRAN
4 - Department of Clinical Sciences, Faculty of Veterinary Medicine, Islamic Azad University, Karaj branch, Karaj, IRAN
5 - Graduated from Faculty of Veterinary Medicine, Islamic Azad University, Karaj branch, Karaj, IRAN

Received: 2016-11-02 Accepted : 2016-11-02 Published : 2010-06-22

Keywords: Multiplex PCR, Bovine viral diarrhea virus, mucosal disease, PI,

Abstract :

Mucosal disease (MD) and bovine viral diarrhea (BVD) are diseases that have no clinical correlations but they have commonviral etiology. In this respect, genotyping of the virus is not performed in Iran yet, after genotyping, control of the disease byusing proper vaccines could be more effective.In this study, serum samples of suspected calves were taken randomly from 20 farms around province of Tehran. Then, twoELISA tests were performed for detection of BVDV antigen. Twenty samples were chosen for RNA extraction as each samplebelonged to one farm. Then, a multiplex PCR was preformed on the basis of 5′ UTR of BVDV genome with positive samplesfor genotyping the virus.In conclusion, 3 samples (15%) were positive for BVD-2 and 20 samples (100%) were positive for BVD-1. All positive andnegative ELISA samples had equal results in RT-PCR. As for detection of BVD-2, phylogenetic analysis and molecular examination is recommended.