Differential Diagnosis of Fasciolosis and Dicrocoeliosis by using Somatic Protein Bands

Subject Areas : clinical veterinary science

عباس Baygan ¹ , نادیا Taiefi Nasrabadi ²

1 - Iranian Institute of Research & Development in Chemical Industries (IRDCI), Jahad Daneshgahi, Tehran, Iran
2 - Department of Basic Science, Faculty of Veterinary Medicine, Islamic Azad University, Karadj Branch, Iran

Received: 2016-11-02 Accepted : 2016-11-02 Published : 2009-12-22

Keywords: Fasciolosis, Clinical Diagnosis, Dicrocoeliosis, Protein Bands, SDS-PAGE,

Abstract :

Dicrocoelium and Fasciola are hepatic duct parasites of different animals that are reported from cattle, buffalo,camel, sheep, goats, wild rabbit and hogs in Iran and many other parts of the world. Studies show that the existenceof this parasites in the livers of final hosts become pathogens.The new technologies in the field of molecularbiology had caused basic changes in the classic researches and indistinguishing parasites. One of these methods isthe somatic proteins identification of the parasites resulted from SDS-PAGE.In an abattoir, adult trematodes were obtained from infested livers of cows, sheep and goats. The different partsof the parasite, such as evacuated materials, excretory and somatic parts can have antigens for diagnosing proteins.There are different method for diagnosing proteins. The best and easiest of these methods is electrophoresis withSDS PAGE. After some processing, some adult termatodes of every host for the expulsion of extra materials waswashed in PBS (pH= 7.4) 3-4 times and the resulting solution was centrifuged in 4oC for 30 min in 12000 G.The results of the present study showed that D. denderiticum protein bands are in 14.4-116 kDa range and theirnumbers is 15. F. hepatica protein bands are in 18-63 kDa and include 7 bands, while F. gigantica in the samerange is 9 bands.Using somatic proteins pattern can be considered as a new paraclinic method for the differential diagnosis ofFascioliasis and Dicrocoeliasis.