Comparison of therapeutic effects of Platelet Rich Plasma Gel and Phenytoin on healing of full thickness skin wound in New Zealand white rabbit
Subject Areas : clinical veterinary science, M Darestani Farahaani 1 , M. E Najafzadeh Khoei, 2 , فریبرز Moayer, 3
1 - Department of Clinical Studies, Karaj Branch, Faculty of Veterinary Medicine.
Islamic Azad University , Karaj, Iran
2 - Graduated of Faculty of Veterinary Medicine, Karaj Branch , Islamic Azad University,
Karaj, Iran
3 - Department of Pathobiology, Faculty of Veterinary Medicine , Karaj Branch, Islamic
Azad University, Karaj, Iran
Keywords: Rabbit, Phenytoin, wound management, Platelet-rich plasma,
Abstract :
Platelets Rich Plasma (PRP) technique, was introduced in 1987 and now is widely in use amongst in skin wound treatment . This study has been compared the effects of platelet-rich plasma and phenytoin on experimental fullthickness wounds in rabbits. A total of 15 healthy, adult male New Zealand rabbits with 1300 g (100 ± g) weight and 5 months (1 ± months) old age, were randomly divided in5 equal groups and were kept in individual cages. Heterologous PRP used in this study, was obtained via the collection of 10 mL blood from a mature New Zealand White male rabbits and centrifuged by two-steps procedure. Anesthesia were induced in rabbits by injecting of appropriate doses of xylazine and ketamine via the IM route , then performed pre-operative consideration ,and fnally a full-thickness wound with dimension of 1.5 × 1.5 cm created on both side of chest in all rabbits . Right and left wounds covered up with four drops of heterologous PRP and 0.5 Cm3 of phenytoin sodium (cream1%), respectively. During the 21-day period of the study, photographic images prepared from all wounds daily and then analyzed of wounds area and healing process, by Scion Image software. Under appropriate anesthesia in days of 3,5,7,14,21 after wound creation, histopathologic specimens were obtained from groups 1-5, respectively. The obtained data were analyzed in U MANN-Withney and T-Test. It was found different signifcance between days 14 to 21 only in wounds area and volume of granulation tissue in wounds treated with PRP v/s wounds treated with phenytoin sodium and there was no signifcant difference in other criteria’s (P <0.05).