Comparison between phytoestrogen Trigonella foenum- gracum L seed and 17 β estradiol, on ovarian and hepatic tissues of female immature three spot gourami
Subject Areas : clinical veterinary scienceطاهره Naji, 1 , P. Shahruzifar, 2 , H. Hosseinzade 3
1 - Department of Basic Sciences, Pharmaceutical Sciences Branch, Islamic Azad
University, Tehran, Iran
2 - Department of Basic Sciences, Pharmaceutical Sciences Branch, Islamic Azad
University, Tehran, Iran.
3 - Research Institute of Fisheries Science, Tehran, Iran
Keywords: liver, Ovary, three spot gourami, 17β estradiol (E2), Trigonella foenum-graecum,
Abstract :
Studies shows that Fenugreek is due to estrogenic compounds phytoestrogenic properties.In this study, it is used Fenugreek seed extract as a phytoestrogen and 17β estradiol (E2) on ovarian and liver tissues of female spot gourami. For this purpose, the fshes were released to chlorine less aquarium water, their weighted average was between 1.5 ± 0.5 g. They were kept for one week in same condition for adaptation with new condition. These experiments were performed in ten groups (two control groups and eight treated groups); each group contained ten immature of female fshes. For 20 days, Fenugreek extract and E2 (following doses 10,20,30,50 mg/kg) were injected separately under dorsal fn, injection method was IM (Inter muscular). After 24 hours, ovary and liver tissues were taken, after passage of texture and staining, the tissues were examined under microscope and were compared with control treatment. Then using the software programs like SPSS and statistical tests like DUNCON and ANOVA, Ovarian and liver tissue samples were compared with different treatments as well as Gonadosomatic and hepatosomatic indices were measured. Signifcant differences in treatment of GSI and HSI treated with Trigonella foenum-graceum L and E2 was observed in control groups. Results showed that increasing the dose of fenugreek extract and E2 GSI, HSI and diameter of oocytes increased but this increase was more signifcant in E2.