Prevalence of antimicrobial resistance, the intI1 and Sul1 genes in Salmonella Enteritidis strains isolated from livestock and poultry

Subject Areas : clinical veterinary science

میترا Salehi, ¹ , پریسا Mobaseri, ² , , F Hosseini ³

1 - Department of Microbiology, Faculty of biology sciences, Tehran north Branch, Islamic Azad University, Tehran, Iran.
2 - Department of Microbiology, Faculty of biology sciences, Tehran north Branch, Islamic Azad University, Tehran, Iran
3 - Department of Microbiology, Faculty of biology sciences, Tehran north Branch, Islamic Azad University, Tehran, Iran

Received: 2016-11-04 Accepted : 2016-11-04 Published : 2014-06-22

Keywords: Salmonella Enteritidis, intI1 gene, sul1 gene, Multidrug resistance,

Abstract :

Bacterial antibiotic resistance has become a worldwide public health problem with direct impact on food safety.Salmonella is an important cause of food-borne gastroenteritis in humans, and diarrhea and sometimes septicemiain animals. Integrons are genetic elements that recognize and capture mobile gene cassettes, which usuallyencode antimicrobial drug resistance determinants. Integrons are usually found in association with transposonsand plasmids. The study included 31 Salmonella Enteritidis isolates collected in Iran, in 2012. The isolates wererecovered from animal sources. All samples were assessed by culture method and standard biochemical tests fordiagnosis of Salmonella strains. After DNA extraction the presence of intI1 and Sul1 genes were examined byPCR. The most common resistance phenotypes were to cefalothin (100%), ampicillin (54/8%), chloramphenicol(51/6%), tetracycline (45/1%), sulfamethoxazole (41/9%), amoxicillin / clavulanate (38/7%). The intI1 gene wasfound in (47%) and (42/8%) and the sul1 gene in (35/2%) and (35/7%) of Salmonella isolates from livestock andpoultry respectively. Integron positive isolates had higher resistance to tetracycline, chloramphenicol, ampicillinsulfamethoxazole and amoxicillin / clavulanate compared with integron negative isolates. The ability of Integronsto integrate resistance gene to antimicrobial agents improves the diffusion of antibiotic resistance.