Histopathologic comparison between specifc Gomori iron staining method and Perl’s Prussian blue staining method in caprine Iron accumulated tissues (Cloisonné kidney)

Subject Areas : clinical veterinary science

, M Nasiri ¹ , , M Sadrkhanlou ²

1 - Department of pathobiology, Faculty of veterinary medicine, Urmia Branch, Islamic Azad University, Urmia, Iran
2 - Faculty of Paramedical Science, Urmia Branch, Islamic Azad University, Urmia, Iran

Received: 2016-11-03 Accepted : 2016-11-03 Published : 2013-03-21

Keywords: Cloisonné kidney, Perl’s Prussian blue, Gomori, specifc staining method,

Abstract :

Deposition of iron particles and pigments may have different effects on affected tissues. For differential diagnosis of each substances deposited in cells and tissues, examiners have to use specifc methods for each material. This diagnosis usually based on the specifc staining methods of affected tissue. Two different specifc Iron staining methods Gomori and Perl’s Prussian blue were compared in this study. Among this survey 860 slaughtered Caprine were investigated histopathlogically and macroscopic Iron pigments deposition grossly found in 8 of these animals. This accumulation was in renal cortical area pertaining to cloisonné kidney disorder. After fxation in formalin buffer 10% solution, samples dyed with two specifc Iron staining methods: Perl’s Prussian blue andgomori. Comparison results showed signifcant differences between quality and quantity of prepared histopathologic samples. For more scientifc investigation, computerized analytic software Scion Image used for comparing prepared images of two different staining methods. The received data used in analytic software SPSS for statistical analysis. The results confrmed exactly more effciency of Perl’s staining method compared to Gomori iron staining method. These superiorities were in more effective coloration of Iron pigments, peripheral cells and more contrast and resolution of stained tissues.