Detection of Rota virus, corona virus, E.coli (k99), cryptosporidium and salmonella in diarrheic calves (up to one month old) in Tehran province farms
Subject Areas : clinical veterinary science, F Moosakhani 1 , آریا Badiei 2 , A.H Asadi 3 , حامد Asadi 4 , علیرضا Shaghayegh 5 , محسن Mozaffari 6
1 - Department of Microbiology, Faculty of Veterinary Medicine, Karaj Branch,
Islamic Azad University, Karaj, Iran.
2 - Department of Clinical Science, Faculty of Veterinary Medicine, Karaj Branch,
Islamic Azad University, Karaj, Iran.
3 - Faculty of Veterinary Medicine, Karaj Branch,
Islamic Azad University, Karaj, Iran.
4 - Animal nutrition Phd Student,Science and Research Branch, Islamic Azad University,
Tehran, Iran
5 - Department of Clinical Science, Faculty of Veterinary Medicine, Karaj Branch,
Islamic Azad University, Karaj, Iran.
6 - Faculty of Veterinary Medicine, Karaj Branch,
Islamic Azad University, Karaj, Iran.
Keywords: Salmonella spp, Cryptosporidium, Rota virus, Corona virus, E.coli (k99),
Abstract :
The aim of the current study was to estimate the prevalence of five major enteropathogens in under 1 month old diarrheic claves in Tehran province.also, the relationship between season with occurrence these Enteropathogens and comparison the results with other geographical regions were evaluated.135 fecal samples from diarrheic calves (under 1 momnth old) collected from 25 farms in Tehran province between April 2010 to June 2011.samples were examined for rotavirus,corona virus,E.coli(k99) and cryptosporidium by using capture Elisa and culturing for samonella spp.salmonella infected samples were serotyped by standard antiserum.Chi-square test was used to determine season association with five enteric pathogens prevalence.totally infection rate from 135 fecal samples , cryptosporidium in 53 samples(39.2%), Rota virus in 43 samples(31.8%),Salmonella spp in 13 samples(9.6%), Corona virus in 12 samples(8.8%) and E.coli (k99) in 8 samples(5.9%) were detected.from 13 salmonella isolated samples,seven of them (5.18%) were typed as salmonella serogroup D and six of them(4.4%) were typed as salmonella serogroup B.the result of this study indicated that cryptosporidium and rotavirus were the most frequently identified pathogens in this study.also season had significant association with salmonella,rota virus and E.coli(k99) prevalence.(p-value<0.05-confident interval (C.I)=95%).