Histopathological evaluation of the healing effects of mesenchymal stem cell transplantation in rat femur defect
Subject Areas : Veterinary pathology
Fatemeh Aghaei
1
,
Samine Mashayekhi
2
,
Saeed Hesaraki
3
1 - Department of Pathobiology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
2 - Department of Pathobiology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
3 - Department of Pathobiology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Keywords: defect, bone, healing, mesenchymal stem cell, hydroxyapatite,
Abstract :
Mesenchymal stem cells (BMSC) can differentiate into the mesodermal lineage. The use of nanocomposite mesenchymal stem cells in the repair of the bone defect of the rat femur. In this study, 36 male Wistar rats were randomly divided into three groups on days 15, 30, and 45. The studied groups include Sham; the defect was left without scaffolding, and the area was closed. HA: The defect was first filled and then closed using hydroxyapatite. Autograft: The defect was filled using pieces of bone from the drilled area and the stem cells (MSCs); that was the same as the control group, but the injection of mesenchymal stem cells was done locally at the time of surgery. In this way, a 5 cm long incision was made in aseptic conditions along the right hind limb on the medial diaphysis of the femur. The periosteum was separated from the diaphysis by a periosteal lifter. A defect with a diameter of 2 mm was created in the femur. Bone samples were fixed, decalcified, and processed for histopathological evaluation on days 30 and 45 to grade lesions and repair rate. The restorative effects of using stem cells were significantly different in all cases compared to other groups. It can be acknowledged that the presence of mesenchymal stem cells positively affects the healing process and shows satisfactory bone strength. Mesenchymal stem cells can be widely used in orthopedic surgery and tissue engineering.