Evaluation of the immunization effects of Parascaris equorum antigen by using of Counter immunoelectrophoresis method

Subject Areas : clinical veterinary science

fatemeh Forohi ¹ , Hadi Zamani ² , Sajjad Yazdansetad ³ , Reza Najafpur ⁴ , Elaheh Shams ⁵ , Hossein vazini ⁶

1 - Assistant Professor, Shahre Qods Branch, Islamic Azad University, Tehran, Iran.
2 - Assistant Professor, Shahre Qods Branch, Islamic Azad University, Tehran, Iran.
3 - Ph.D., Golestan University of Medical Sciences, Gorgan, Iran.
4 - Ph.D., Qom Branch, Islamic Azad University, Qom, Iran.
5 - Young Researchers and Elite Club, Falavarjan Branch, Islamic Azad University, Isfahan, Iran.
6 - Assistant Professor, Nursing Department, Basic Sciencess Faculty, Hamadan Branch, Islamic Azad University, Hamadan, Iran.

Received: 2017-06-13 Accepted : 2018-01-22 Published : 2018-08-23

Keywords: Immunogenicity, Parascaris equorum, Counter immunoelectrophoresis method,

Abstract :

Introduction: Parascaris equorum, is an important cosmopolitan nematode parasite of foals. The preparation of antigen is a basic practice to adjust a new sero-diagnostic method and also for produce vaccine. This study is aimed to evaluate the antigenic effects of Parascaris antigens by Counter immunoelectrophoresis (CIEP).Materials and Methods: In current work the intestine of the horses were longitudinally opened and the worm were collected. The genus and species of the worms were assessed by morphologic and morphometric techniques and stored in -20 ºC until to use. Worms were crushed in PBS method and then sonicated. Antigens in different concentrations alone or with adjuvant were injected to the rabbit and the production of Antibodies was analyzed by CIEP method.Results: The effect of antigen isolated from Parascaris equorum parasite in the production of antibodies was significantly in comparison with the negative control group. Also, indicated that production of antibody was increased by use of adjuvant with antigen. The productions of antibody in rabbits that receive antigen two times per a week were more than ones, which receive one time per a week.Conclusion: Total antigens of Parascaris equorum parasite is an anti-geneticaly and stimulating agent. These antigens could be a candidate for provision diagnostic kits and also for produce vaccine.