The Biological Effects Pesticide of Chlorpyrifos On the development of Balb/C Mouse Embryos Third to Sixth Days Of Pregnancy

Subject Areas : clinical veterinary science

safoora saffari ¹ , Parvin Torabzadeh ² , Saba Rastgar gharahshiran ³

1 - Islamic Azad University, karaj
2 - Islamic Azad University, karaj
3 - Islamic Azad University, karaj.

Received: 2015-09-14 Accepted : 2016-05-05 Published : 2017-02-19

Keywords: Chlorpyrifos, Mouse embryo, embryo weight, placental weight, Agricultural pesticides,

Abstract :

Introduction: Chlorpyrifos is one of organophosphate Insecticides and had studied in recent years because it has many complications devastating on skin, nervous, respiratory and digestive systems. But It is effects on the embryo especially during pregnancy has not been reviewed, . Therefore in study The Biological effects pesticide of Chlorpyrifos on the development of Balb/C mouse embryos Third to Sixth days of pregnancy.Material and Method: In this study 90 female Balb/C mouse were randomly divided into 6 equal groups acontrol group (non- injection) and witess (injection of saline) and 4 experimental groups. A lethaldose LD50 was determined in condition of 25/32 ml/kg.bw in vivo and selected dose for injection 0/4 ml/kg.bw. Injection was done on the Third to Sixth days of pregnancy days by enema. Then the mouse were sacrificed on day 15 of pregnancy.For security of results above experiences was repeated three times. Data was checked with SPSS17 software with ANOVA and Duncan test subjest to (P