Study of the preventive effects of cetronelix on blood-testis barrier and spermatogenesis injuries caused by chemotherapy wich cisplatin in adult Balb/C mice

Subject Areas : Veterinary Clinical Pathology

Received: 2013-05-01 Accepted : 2013-10-12 Published : 2013-05-22

Keywords: Spermatogenesis, Blood-testis barrier, Chemotherapy,

Abstract :

Abstract   Chemotherapy agents, in addition to cancer cells, target all dividing cells in clouding spermatogenic cells. These cells could be protected from the determintal effects of chemotherapy agents in addition of FSH and LH secretion using GnRH antagonist (cetrolix) . In the present study 30 male balb/c mice were randomly divided into 3 groups of control (receiving no treatment), experimental 1 (receiving a 2.5mg/kg/bw dose of cisplatin as intraperitoneal injection for 5 days) and experimental 2 (a group that received GnRH antagonist in addition to cisplatin). Five week safter the last injection, the mice were killed by cervical dislocation and testicular specimens were prepared for light and electron microscopy. The data were analyzed using ANOVA and Tukey´s test and p<0.05 is considered as significant. Morphometric results showed that spermatogenesis index (SI) in control and experimental 1 groups were respectively 24.43±9.22 and 1.52±1.39,respectively the number of sertoli cells in control and experimental 1 groups were 5.50±0.52 and 8.61±0.80,respectively showing a significant reduction of SI and increasing of sertoli cells in experimental 1 group (p<0.05). Electron microscopy revealed that in experimental 1 group, blood-testis barrier was irregular and sertoli cells were separated from basal lamina. In experimental 2 group, cetrorelix almost prevented such changes.  It can be concluded that cetrorelix could almost prevent the detrimental effects of chemotherapy on germinal epithelium. 

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