Survey of primultaneous Contamination with CIAV and infection with AI and ND in broiler chicken of Chaharmahal - va - Bakhtiari provence
Subject Areas :
Veterinary Clinical Pathology
ezzatollah fathihafshanjani
1
1 - دانشگاه آزاد اسلامی، واحد شهرکرد، دانشکده دامپزشکی، استادیار بخش بیماریهای طیور، شهرکرد، ایران.
Received: 2013-04-17
Accepted : 2013-10-21
Published : 2013-05-22
Keywords:
PCR,
Histopathology,
Infectious Anemia Virus,
Chaharmahal Va Bakhtiyari,
Abstract :
Abstract
The agent of chicken infectious anemia (CIA) belongs to Circoviridae. CIA can cause severe anemia, immunosuppression and high sensitivity to bacterial, viral, parasitic and fungal pathogens. The aim of this study was to investigate simultaneous infection of CIA with Newcastle disease (ND) and Avian influenza (AI) in broiler chickens of Chahrmahal-va-Bakhtiari province. For this purpose, in 2011, from 14 broilers chicken flocks at 2-7 weeks old age, with confirmed ND and AI thymus and bursa of fabricious were sampled for amplification of 1390 base pair fragment of VP1 of CIA virus. Histopathologic the result of PCR showed that 4 flocks (28.57%) were one hundred percent infected with CIA. examination approved the results of PCR. This study showed high simultaneous infection rate of CIA with ND and AI that causes high mortality in these flocks. Therefore, this may be related to immunosuppressant effect of CIA, alone or with other infections.
References:
ﻋﻤﺎدی، آ. (1383). ﺟﺴﺘﺠﻮی ژﻧﻮم وﻳﺮوس ﻛﻢﺧﻮﻧﻲ ﻋﻔﻮﻧﻲ ﺟﻮﺟﻪ ﺑﻪ روش PCR درﺟﻮﺟﻪﻫﺎی ﮔﻮﺷﺘﻲ اﺳﺘﺎن ﭼﻬﺎر ﻣﺤﺎل و ﺑﺨﺘﻴﺎری. داﻧﺸﻜﺪه داﻣﭙﺰﺷﻜﻲ، داﻧﺸﮕﺎه ﺷﻬﺮﻛﺮد، ﭘﺎﻳﺎنﻧﺎﻣﻪ شماره 91.
· محمد زاده، پ. (1382). جستجوی ویروس کم خونی عفونی جوجهها در استان کردستان. شرکت داروی والا اندیشان ناب.
Bassami, M.R., Berryman, D., Wilcox, G.E. and Raidal, S.R. (1998). Psittacine beak and feather disease virus nucleotide sequence analysis and its relationship to porcine circovirus plant circoviruses, and chicken anemia virus. Virology, 249(2): 453-459.
Cloud, S.S., Rosenberger, J.K. and Lillehoj, H.S. (1992b). Immune dysfunction following infection with chicken anemia agent and infectious bursal disease virus. 2. Alterations of in vitro lymphoproliferation and in vivo immune responses. Veterinary Immunopathology, 34: 353-366.
Goryo, M., Suwa, T., Umemura, T., Itakura, C. and Yamashiro, S. (1989a). Histopathology of chicks inoculated with chicken anemia agent (MSB1-TK5803 strain). Avian Pathology, 18: 73-89.
Hejazi, A.M., Abdallahi, F.M., Abd-El Samie, L.K. and Nazim, A.A. (2010). Chicken Infectious Anemia Virus in Broilers and Laying Hensin Sharkia Province Egypt. Journal of American Science, 6(9): 52-59
Imai, K. and Yuasa, N. (1990). Development of a micro test method for serological and virological examinations of chicken anemia agent. Japanes Journal of Veterinary Sciences, 52: 873-875.
Jordan, F., Pattison, M., Alexander, D. and Faragher, T. (2001). Poultry Disease. 5th ed., Londan: W.B. Sunders, pp: 352-356.
Lucio, B., Schat, K.A. and Shivaprasad, H.L. (1990). Identification of the chicken anemia agent, reproduction of the disease and serological survey in the United States. Avian Disease, 34: 146-153.
Mahzounieh, M., Karimi, I. and ZahraeiSalehi, T. (2005). Serologic evidence of chicken infectious anemia in commercial chickens flocks in Shahrekord, Iran. International Journal of Poultry Science, 4(7): 500-503
Moemen, A.M. (2010). Chicken infectious anemia status in commercial broiler chickens flocks in Assiut-upper Egypt, occurrence, molecular analysis using PCR-RFLP and apoptosis effect on affected tissues. International Journal of Poultry Science, 9(6): 591-598
Natesan, S., Kataria, J.M., Dham, K., Rahul, S. and Baradhwaj, N. (2006). Biological and molecular characterization of Chicken Infectious Anemia virus isolates of Indian origin. Virus Research, 118: 860-878.
Nogueira, E.O., Brentano, L. and Ferreira, A.J.P. (2005). A VP3/VP1 gene polymerase chain reaction assay for detection of chicken anemia virus in broiler samples. Brazilian Journal of Veterinary and Animal Science, 57(2): 131-140.
Otaki, Y.K., Saito, M.T. and Namura, Y. (1991). Detection of antibody to chicken anemia agent acomparison of three serological tests. Avian Pathology, 20: 315-324.
Saif‚ Y.M.‚ Barnes, H.J.‚ Glisson, J.R.‚ Fadly‚ A.M., Mcdougald, L.R. and Swayne, D.E. (2003). Disease of poultry. 11th ed., USA: Iowa State Press‚ Blackwell, pp: 181-202.
Toro, H., Ramirez, A.M. and Larenas, J. (1997). Pathogen city of chicken anemia virus (isolate 10343) for young and older chickens. Avian Pathology, 26(3): 485-499.
Yassir, M.E., KunQian, W.J., Pingping, W. and Aijian, Q. (2011). Molecular epidemiology of chicken anemia virus in commercial farms in China. Virology Journal, 8: 145.
Yuasa, N., Taniguchi, T. and Yoshida, I. (1979). Isolation and some characteristics of an agent inducing anemia in chicks. Avian Disease, 23: 366-385.