Detection of Infectious Bronchitis Virus (4/91 type) in Broiler Chickens in Chahrmahal-va-bakhtiyari Province

Subject Areas : Veterinary Clinical Pathology

مجید Gholami-Ahangaran ^{1 *} , A.M Shoushtari ² , عباس Doosti ³ , E.A Fathi Hafshejani ⁴ , نوشا Zia-Jahromi ⁵

1 - Department of Poultry Diseases, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
2 - Poultry Diseases Department, Razi Vaccine & Serum Research Institute, Karaj, Iran
3 - Department of Microbiology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
4 - Department of Poultry Diseases, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
5 - Department of Microbiology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran

Received: 2012-01-20 Accepted : 2012-11-21 Published : 2012-08-22

Keywords: PCR, broiler chicken, Infectious Bronchitis, 4/91 (793/B), Chahrmahal-va-bakhtiyari province,

Abstract :

Infectious bronchitis (IB) disease is a viral contagious respiratory disease. The causing agent of this disease has several serotypes. In this study, 4/91 type of Infectious bronchitis (IB) was identified. For this, tracheal samples were taken from 18 broiler chickens flocks having respiratory signs suspected to IB disease with one percent mortality in day. After RNA extraction from tissue samples in one step RT-PCR reaction, a fragment of S1 gene was amplified by common primers for all IB viruses. Then RT-PCR product was amplified for identification of 4/91(793/B) type by type specific primers in Nested-PCR. Results showed, 11 out 18 flocks (61.1%) were infected to IB that 45.45% of IB infected flocks were infected to 4/91 type. Therefore it seems 4/91 type of IB has role in forming and complexing of respiratory signs in broiler chickens suffering to respiratory syndrome in Chahrmahal-va-bakhtiyari province and it is necessary to give a suitable controlling strategy for prevention of 4/91 infection.

References:

1. اکبری آزاد، گ.، وصفی مرندی، م. و کیوانی، ح. (1383): جداسازی و شناسایی مولکولی ویروس­های برونشیت عفونی طیور در مرغداریهای صنعتی ایران. مجله دانشکده دامپزشکی دانشگاه تهران، 59(3): صفحات: 264-259.


2. غلامی آهنگران، م.، چرخکار، س.، شوشتری ع.، بزرگمهری فرد م.ح.، و عشرت آبادی ف. (1387): شناسایی مولکولی و تعیین تیپ ویروس برونشیت عفونی در موارد سندرم تنفسی جوجه­های گوشتی در استان اصفهان. مجله علوم دامپزشکی ایران، 5 (3): صفحات: 476-469.


3. Adzhar, A.B., Show, K.B., Britton, P., and Cavanagh, D. (1995): Avian infectious bronchitis virus: differences between 793/B and other strains. Veterinary Record. 136(27):548.


4. Adzhar, A., Gough, R.E., Haydon, D., Shaw, K., Britton, P., and Cavanagh, D. (1997): Molecular analysis of the 793/B serotype of infectious bronchitis virus in Great Britain. Avian Pathology. 26:625-640.


5. Aghakhan, S.M., Abshar, N., Rasoul Nejad Fereidouni, S., Marunesi, C., and Khodashenas, M. (1994): Studies on avian viral infections in Iran. Archive del Institut Razi. 44/45:1-10.


6. Binns, M.M., Boursnell, M.E.G., Cavanagh, D., Pappin, D.J.C., and Brown, T.D.K. (1985): Cloning and sequencing of the gene encoding the spike protein of the coronavirus IBV. Journal of General Virology. 66:719-726.


7. Cavanagh, D., Mawditt, K., Britton, P., and Naylor, C.J. (1999): Longitudinal field studies of infectious bronchitis virus and avian pneumovirus in broiler using type specific polymerase chain reaction. Avian Pathology. 28:593-605.


8. Cavanagh, D. and  Gelb, J. Infectious bronchitis. In: Saif, Y.M. Fadly, A.M. Glisson, J.R. McDougald, L.R. and Nolan, L.K. Swayne, D.E. (2008): Diseases of Poultry. 12th ed. Blackwell Publishing, Iowa State University Press, USA, Ames, pp: 117-135.


9. Mahdavi, S., Tavasoly, A., Pourbakhsh, S.A., Momayez, R. (2007): Experimental histopathologic study of the lesions induced by serotype 793/B (4/91) infectious bronchitis virus. Archives of Razi Institute, 62(2): 15-22.


10. Meulemans, G., Boschmans, M., Decaesstecker, M., van den Berg, T.P., Denis, P., and Cavanagh, D. (2001): Epidemiology of infectious bronchitis virus in Belgian broilers: a retrospective study1986-1995. Avian Pathology. 30:411-421.


11. Nouri, A., Assasi, K., and Seify-abad Shapouri, M.R. (2003): Field study of infectious bronchitis virus in broiler using type-spscific RT-PCR. Archives of Razi Institute. 55:1-10.


12. Ramneek, R., Mitchel, N.L., and Mcfarlane, R.G. (2005): Rapid detection and charecterisation of IBV from New Zealand using RT-PCR and sequence analysis. New Zealand Veterinary Journal. 53(6):457-461.


13. Seify-abad Shapouri, M.R., Mayahi, M., Charkhkar, S., and Assasi, K. (2002): Serotype identification of recent Iranian isolates of infectious bronchitis virus by type specific multiplex RT-PCR. Archives of Razi Institute. 53:79-85.


14. Vasfi Marandi, M., and Bozorgmehri Fard, M.H. (2000): Isolation and identification of infectious bronchitis virus in chicken in Iran. Proceeding of the 21^st World^,s Poultry Congress, Aug:20-25, Montreal, Canada.