Prevalence of bovine viral diarrhea disease investigated with indirect ELISA method in dairy Holstein cows of Tabriz region

Subject Areas : Veterinary Clinical Pathology

افشین  Davasaz Tabrizi ¹ (Department of Clinical Sciences, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran)
پیمان  Zare ² (Department of Pathobiology, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran)
یوسف  Davoudi ³ (Veterinary Group, Sarab Branch-Islamic Azad University, Sarab, Iran)
صمد  Mosaferi ⁴ (Department of Clinical Sciences, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran)
مهران  Alamdari ⁵ (General Veterinary Office of East Azerbaijan Province)

Received: 2010-10-02 Accepted : 2011-02-15 Published : 2011-05-22

Keywords: cattle, Elisa, Diarrhea,

Abstract :

Bovine viral diarrhea suppresses the immune system and makes animals susceptible to other diseases. Considering that the most common form of this disease is the chronic form, it is not considered seriously in our country. Since in many dairy herds there are many infertility cases without any specific cause and there are no vaccinations or control programs against bovine viral diarrhea in dairy farms of Tabriz, the need to investigate the prevalence of this disease in dairy farms across the region becomes obvious. Venous blood samples were obtained from 508 Holstein cows of dairy of Tabriz. Sera were maintained until testing at temperature of -20°C. Serum samples were tested using indirect ELISA and commercial kits for inspecting specific antibodies of bovine viral diarrhea. From the total of 508 cows that were studied, 95 cases (18.7 percent) had antibodies against bovine viral diarrhea. 

References:

1. حاجی حاجیکلایی، م.ر. و صیفی آبادشاپوری، م.ر. 1386. بررسی سرولوژیکی آلودگی به اسهال ویروسی گاو در گاوهای اهواز، تحقیقات دامپزشکی، شماره 245، صفحه: 21.


2. جفری، اچ.آرتور. 1379. تولید مثل ومامایی دامپزشکی. ترجمه علوی شوشتری، م. جلد دوم. صفحات: 963-958.


3. صدیقی­نژاد، ص. 1375. بررسی اسهال ویروسی گاو، بیماری مخاطی در ایران. پژوهش و سازندگی، شماره 30، صفحه : 127.


4. کارگر موخر، ر.، اهورائی، پ.، حسامی، م.، تقی‌پور بازرگانی، ت.، غلامی، م.ر.، خدمتی، ک. و همکاران. 1374. گزارش وجود و میزان شیوع بیماری BVD/MD در گاوداری­های اطراف تهران. پژوهش و سازندگی، شماره 28، صفحات: 116- 112.


5. مرشدی، ا.، محمودیان، ع.، دلیر نقده، ب. و حاجی­زاده، ج. 1383. بررسی سرم شناسی میزان شیوع آلودگی گاوها به BVDV با آزمون الایزا و مقایسه استفاده از الایزای شیر و سرم خون در ارومیه. تحقیقات دامپزشکی، شماره 235، صفحه: 227.


6. ممتاز، ح.و همت زاده، ف. 1382. تعیین میزان عفونت پستی ویروسی در گاوداری­های شهرستان شهرکرد. پژوهش وسازندگی، شماره 59، صفحات: 91-86.


7. همت‌زاده، ف.، کجوری، غ.، کارگر موخر، ر.، روحانی، م. 1380. بررسی سرمی بیماری اسهال ویروسی گاوان در استان چهارمحال بختیاری. مجله تحقیقات دامپزشکی، دوره 56، شماره 3، صفحه: 92-85.


8. Bitsch, V., Hansen, K., Ronsholt, L. 2000. Experiences from the Danish Programmed for eradication of bovine virus diarrhea (BVD) 1994- 1995 with special reference to legislation and causes of infection. Veterinary Microbiology, 77:137-143.


9. Browline, J. 2002. Bovine virus diarrhea virus: Pathogenesis and control. In: Recent developments and Perspective in Bovine Medicine. Keynote lectures in xxll world Buiatrics congress, 18-23, August 2002, Hannover, Germany. Edited by Martin, Kaske Henner, Scholz and Martin Holtershinken, 24-31.


10. Ferrari, G., Scicluna, M.T., Bonvicini, D., Gobbi, C., Della Verita, F., Valentini, A. and Autorino, G. L. 1999. Bovine virus diarrhea (BVD) control program in an area in the Rome province (Italy). Veterinary Microbiology, 64:237-245.


11. Grom, J. and Barlic Maganja, D. 1999. Bovine Viral Diarrhea (BVD) infectious- control and eradication program in breeding herds in Slovenia. Veterinary Microbiology, 64:259-264.


12. Harkness, J.W., Sands, J.J. and Richard, M.S. 1978. Serological studies of mucosal disease in England and Wales, Research Veterinary Science. 24:98-103.


13. Houe, H., Meyling, A. 1991. Prevalence of bovine virus diarrhea (BVD) in Danish dairy herds and estimation of incidence of infection in early pregnancy. Preventive Veterinary Medicine, 11:9-16.


14. Loken, T., Krogsrud, J., Bjerkas, I. 1991. Pestivirus infection in Norway. Serological investigations in cattle, sheep and pigs. Acta Veterina Scandinavia, 32:27-34.


15. Paisley, L.G., Wells, S., Schmitt, B.J. 1996. Prevalence of bovine viral diarrhea antibodies in 256 U.S. Cow-calf operations: A survey. Theriogenology, 46:1313-1323.


16. Robert, F.K. 2001. Viral Disease of cattle. Second edition. Iowa state, University press, USA. pp. 113-126, 159-170.


17. Robhun, W.C., Guard, C., Richards, C.M. 1995. Disease of Dairy cattle. 1st Ed. Williams and Wilkins. London. pp. 80-82, 197-208.


18. Rodostits, O.M., Gay, C.C., Blood, D.C. and Hinchcliff, K.W. 2000. Veterinary Medicine. W.B. Saunders, London. 9th Edition. p: 1085-1105.


19. Rufenacht, J., Schaller, P., Audige, L., Kuntti, B., Kupfer, U. and Peterhans, E. 2001. The effect of infection with bovine viral diarrhea virus on the fertility of Swiss dairy cattle. Theriogenology, 56:199-210.


20. Synge, B.A., Clark, A.M., Moar, J.A.E., Nicolson, J.T., Nettleton, P.F. and Herring, J.A. 1999. The Control of bovine virus diarrhea virus in Shetland. Veterinary Microbiology, 64:223-229.


21. Talebkhan Garoussi, M., Haghparast, A., Estajee, H. 2008. Prevalence of bovine viral diarrhea virus antibodies in bulk tank milk of industrial dairy cattle herds in suburb of mashhad-Iran. Preventive Veterinary Medicine, 84:171-176.


22. Taylor, L.F. and Rodwell, B.J. 2001. Outbreak of fetal infection with bovine pestivirus in a central Queensland beef herd. Australian Veterinary Journal, 79: 682-685.


23. Valle, P.S., Martin, S.W., Tremblay, R. and Bateman, K. 1999. Factors associated with being a bovine virus diarrhea (BVD) seropositive dairy herd in the M [oslash] re and Romsdal country of Norway. Preventive Veterinary Medicine, 40:165-177.