Serologic investigation of the prevalence of Equine infectious anemia virus in Tabriz area
Subject Areas : Veterinary Clinical Pathology
علی Hassanpour
1
(Assistant Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Islamic Azad university- Tabriz Branch, Tabriz, Iran)
A.P Rezaei Saber
2
(Assistant Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Islamic Azad university- Tabriz Branch, Tabriz, Iran)
فرهاد Mosakhani
3
(Assistant Professor, Department of Pathobiology, Faculty of Veterinary Medicine, Islamic Azad university, Karaj Branch-Karaj, Iran)
Keywords: Tabriz, Equine infectious anemia, Serologic contamination, EILISA,
Abstract :
Abstract Equine infectious anemia (EIA) is caused by the equine infectious anemia virus (EIAV), which infects the animals in the family Equidae and is classified in the subfamily Lentivirinae of the family Retroviridae. The disease leads to fever, hemolytic anemias, icterus, depression and chronic weight loss. This study was conducted on 287 horses (200 male and 87 female) in Tabriz area in Iran in order to determine the seroprevalence of equine infectious anemia. Blood was collected from all horses and the sera were analyzed for the presence of antibodies to equine infectious anemia virus (EIAV) using an ELISA test with IDEXX kit. The mean of Optical Density (OD) was 0.372± 0.016. In these samples the lowest and highest levels were 0.273 and 0.511 respectively. There was no positive case according to the observed results. Mean OD was 0.381± 0.014 in male horses and 0.387±0.010 in female horses, and there was no significant difference between the two groups (P= 0.356). The horses were studied in four age groups: 1-3 (54 horses), 3-6 (125 horses), 6-9 (70 horses) and over 9 years old (38 horses). Mean OD was 0.373±0.011, 0.381±0.012, 0.383±0.009, 0.388±0.017 in these groups respectively. The results revealed that none of the horses were positive for antibodies to EIAV.