Detection of Mycoplasma synoviae in clinical samples by VlhA-PCR method

Subject Areas : Veterinary Clinical Pathology

حسین Ansari ¹ , S.A Pourbakhsh ² , نریمان Sheikhi ³ , M.H Bozorgmehri Fard ⁴ , عباس Ashtari ⁵

1 - Ph.D Student of Poultry Diseases, Faculty of Specialized Veterinary Sciences, Islamic Azad University- Science & Research Branch, Tehran, Iran
2 - Department of Microbiology, Faculty of Specialized Veterinary Sciences, Islamic Azad University- Science & Research Branch, Tehran, Iran
3 - Department of Clinical Science, Faculty of Specialized Veterinary Sciences, Islamic Azad University- Science & Research Branch, Tehran, Iran
4 - Department of Clinical Science, Faculty of Specialized Veterinary Sciences, Islamic Azad University- Science & Research Branch, Tehran, Iran
5 - Mycoplasma Reference Lab. of Razi Vaccine and Serum Research Institute, Karadj, Iran

Received: 2009-11-12 Accepted : 2010-05-29 Published : 2010-02-20

Keywords: PCR, Clinical samples, Mycoplasma synoviae, VlhA gene,

Abstract :

As one of the major pathogens of avian species, Mycoplasma Synoviae causes significant economic losses to the poultry industry. The main purpose of this study was to detect Mycoplasma Synoviae in clinical samples using the VlhA-PCR method. For serological screening test, 373 serum samples were collected from 25 breeder farms and rapid serum agglutination test conducted which revealed that 143 samples equivalent to 19 breeder farms were positive. For VlhA-PCR assay, 20 of the previously mentioned breeder farms were selected and sterile swab were collected from the palatine cleft, trachea, air sacs and lungs. Three swabs from 3 birds were placed in a test tube containing 1 ml of PBS and transferred to the laboratory for PCR test. Specific primers for VIhA gene were employed in this study. The PCR product from specific primers showed 350-400 bp for all field isolated on electrophoresis gel in 8 farms. VlhA-PCR with high sensitivity could be employed in definitive diagnosis of Mycoplasma Synoviae infection in the laboratory.

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