Effect of three methods of estrus synchronization: Osynch, Co-Synch and Prostaglandin on the fertility of subfertile dairy cows

Subject Areas : Veterinary Clinical Pathology

habib cheraghi ¹ , khosro Parsaeimehr ² , ali hosseinkhani ³ , sadegh alijani ⁴ , hossein daghighkia ⁵

1 - کارشناس ارشد واحد دامپروری ایستگاه تحقیقاتی و آموزشی خلعت پوشان دانشکده کشاورزی، دانشگاه تبریز، تبریز، ایران.
2 - کارشناس ارشد واحد دامپروری ایستگاه تحقیقاتی و آموزشی خلعت پوشان دانشکده کشاورزی، دانشگاه تبریز، تبریز، ایران.
3 - استادیار گروه علوم دامی، دانشکده کشاورزی، دانشگاه تبریز، تبریز، ایران.
4 - دانشیار گروه علوم دامی، دانشکده کشاورزی، دانشگاه تبریز، تبریز، ایران.
5 - دانشیار گروه علوم دامی، دانشکده کشاورزی، دانشگاه تبریز، تبریز، ایران.

Received: 2014-09-21 Accepted : 2015-04-06 Published : 2015-02-20

Keywords: dairy cow, Ovsynch, Prostaglandin, pregnancy rate, Co-Synch,

Abstract :

This study was performed to compare the effect of using 3 different estrus synchronization methods (Ovsynch, Co-Synch and Prostaglandin) on the conception rate of subfertile dairy cows. Fifty one subfertile Holstein dairy cows with parity ranging from 1 to 7 were enrolled in this study. Cows were allocated to three groups Ovsynch (n=10), Co-Synch (n=27) and Prostaglandin (n=14). Cows in the Ovsynch group underwent a standard Ovsynch protocol (GnRH analogue on Day 0, PGF2α analogue on Day 7 and GnRH analogue on Day 9), then they were artificially inseminated approximately 16 hours after the second GnRH treatment. The same procedure was done for cows in Co-Synch group, but artificial insemination took place immediately after the last injection. Cows in prostaglandin group underwent a PG protocol (PGF2α, on Day 0 and 12), and artificial insemination was done about 72 hours after the second PGF2α injection. Pregnancy diagnosis was performed 60 days after insemination via rectal examination. By using logistic procedure of SAS 9.1 software, the effects of treatment, season, parity, animal, age and open days were analyzed. The analysis of variables related to animal and the measured effects on pregnancy proved that none of the evaluated parameters were significant. Hence, we cannot declare which of the estrus synchronization methods is suitable to increase pregnancy rate. Further studies with more replicates are required to choose the best method.

References:

• · رستگارنیا، ع. و انورى، ه. (1389). بررسى تاثیر سیدر در پروتکل همزمان‌سازى فحلى و تلقیح در زمان ثابت (CIDR -synch) بر روی میزان آبستنی گاوهای شیری. مجله پژوهش‌های بالینی دامپزشکی، سال اول، شماره چهارم، صفحات: 252-243.
  
  
  
  • Alkar, A., Tibary, A., Wenz, R.L., Nebel J.R. and Kasimanickam, R. (2011). Presynchronization with GnRH 7 days prior to resynchronization with CO-Synch did not improve pregnancy rate in lactating dairy cows. Theriogenology, 76: 1036-1041.
  
  
  • Forro, A., Tsousis, G., Beindorff, N., Sharifi, R., Jakel L. and Bollwein, H. (2012). Combined use of Ovsynch and progesterone supplementation after artificial insemination in dairy cattle. Journal of Dairy Science, 95: 4372-4381.
  
  
  • Geary, T.W., Whittier, J.C., Downing, E.R., LeFever, D.G., Silcox, R.W., Holland, M.D., et al., (1998). Pregnancy rates of postpartum beef cows that were synchronized using Syncro-Mate-B or the Ovsynch protocol. Journal of Animal Science, 76: 1523-1527.
  
  
  • Geary, T.W., Whittier, J.C., Hallford, D.M. and MacNeil, M.D. (2001). Calf removal improves conception rates to the Ovsynch and CO-Synch protocols. Journal of Animal Science, 79: 1-4.
  
  
  • Karami Shabankareh, H., Zandi, M. and Ganjali, M. (2010). First service pregnancy rates following post-AI use of HCG in ovsynch and heatsynch programs in lactating dairy cows. Reproduction in Domestic Animals, 45: 711-716.
  
  
  • Kasimanickami, R., Cornwell, J.M. and Nebel, R.L. (2005). Fertility following fixed-time AI or insemination at observed estrus in ovsynch and heatsynch programs in lactating dairy cows. Theriogenology, 83:2550-59.
  
  
  • Kim, H.I., Suh, G.H. and Son, D.S. (2003). A progesterone –based timed AI protocol more effectively prevents premature estrus and incomplete luteal regression thon an ovsynch protocol in lactating Holstein cows. Theriogenology, 16: 223-224.
  
  
  • SAS. (2003). Statistical analysis system: A User’s Guide. Version 9.1, SAS Institute Inc., Cary, NC.
  
  
  • Stevenson, J.S., Pursley, J.R., Garverick, H.A., Fricke, P.M., Kesler, D.J., Ottobre, J.S., et al. (2006). Treatment of cycling and noncycling lactating dairy cows with progesterone during ovsynch. Journal of Dairy Science, 89: 2567-2578.
  
  
  • Yilmazbas-Mecitoglu, G., Karakaya, E., Keskin, A., Gumen, A., Koc, V. and Okut, H.  (2014). Comparison of synchronisation and fertility after different modifications of the ovsynch protocol in cyclic dairy cows. Actaveterinaria Hungarica, 62: 64-73.
  
  
  • Youssefi, R., Vojgani, M., Gharagozlou, F. and Akbarinejad, V. (2013). More male calves born after Presynch-Ovsynch protocol with 24-hour timed AI in dairy cows. Theriogenology, 79: 890-894.