The effect of endurance swimming exercise on the occurrence of apoptosis in experimental diabetic myopathic rats
Subject Areas :
Veterinary Clinical Pathology
Received: 2009-01-17
Accepted : 2009-09-28
Published : 2010-02-20
Keywords:
Rat,
Apoptosis,
Diabetic myopathy,
Endurances swimming exercise,
Abstract :
Diabetic myopathy is the main cause of cell injury in the muscular system of diabetic patients. The purpose of this study was to evaluate the effect of swimming exercise on the occurrence apoptosis in diabetic myopathy. In this study, 56 Wistar rats with approximate age of 12 weeks and body weight of 250-300 gr were randomly allocated into two equal groups of 28 rats. Diabetes was induced in both groups by intraperitoneal injection of streptozocin (50 mg/kg). The treatment group was kept in normal management and dietary conditions for 12 weeks with forced swimming, an hour every day, 5 days a week. The control group was kept in similar management and dietary condition without any physical activity. After 12 weeks, muscle tissue samples were collected from the gastrocnemius muscle in both groups and following fixation in 10 % buffered formalin, 5-6 micron tissue sections were prepared and stained with hematoxylin and eosin and TUNEL methods.Histopathological study of representative sections in control group showed apoptosis and necrosis in myocytes. Mild apoptotic and necrotic changes in treatment group were observed. Mean differences of histopathological changes between the experimental groups were significant p<0.001.The results of the present study indicated that swimming is capable of reducing pathologic changes and inducing partial improvement of muscular insults in diabetic myopathy.
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