PCR assays for detection of Toxoplasma gondii infection in Iranian camels (Camelus dromedarius) of Yazd province
Subject Areas :
Veterinary Clinical Pathology
alireza sazmand
1
,
mousa tavassoli
2
,
bijan Esameilnejad
3
,
zahra Asadollahi
4
,
ali Kazemnia
5
,
seyyedhossein Hekmatimoghaddam
6
1 - مربی، گروه کشاورزی، دانشگاه پیام نور یزد، یزد، ایران.
2 - استاد، گروه پاتوبیولوژی، دانشکده دامپزشکی ارومیه، ارومیه، ایران.
3 - استادیار، گروه پاتوبیولوژی، دانشکده دامپزشکی ارومیه، ارومیه، ایران.
4 - دانشجوی دکتری، گروه پاتوبیولوژی، دانشکده دامپزشکی دانشگاه تهران، تهران، ایران.
5 - تکنسین آزمایشگاه، گروه پاتوبیولوژی، دانشکده دامپزشکی ارومیه، ارومیه، ایران.
6 - استادیار، گروه علوم آزمایشگاهی، دانشکده پیراپزشکی، دانشگاه علوم پزشکی و خدمات بهداشتی درمانی شهید صدوقی یزد، یزد، ایران.
Received: 2013-12-24
Accepted : 2014-06-24
Published : 2014-05-22
Keywords:
Iran,
camel,
Toxoplasma gondii,
polymerase chain reaction,
Abstract :
The protozoan parasite Toxoplasma gondii is one the most important zoonotic pathogens. Therefore, in the current pilot study a total of 50 Iranian one-humped camels of both sexes and different ages that were kept by local farmers in Yazd province of Iran were tested for Toxoplasma gondii infection. Whole blood samples were investigated by PCR assay using B1 gene. The results revealed that none of the tested camels were infected with Toxoplasma gondii. The current pilot study is the first attempt for detection of Toxoplasma gondii in camels in Iran by PCR method. Further studies in different regions of the country seem necessary to outline the importance of the disease. Also, experimental infection of camels with Toxoplasma gondii is recommended to study the course of the disease.
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