Molecular diversity of Pasteurella multocida isolated from cattle and buffaloes in East Azerbaijan province based on restriction endonuclease analysis

Subject Areas : Veterinary Clinical Pathology

jalal shayegh ^{1 *} , alireza monadi ² , jalil Dolgari Sharaf ³

1 - دانشگاه آزاد اسلامی، واحد شبستر، استادیار گروه دامپزشکی، شبستر، ایران.
2 - دانشگاه آزاد اسلامی، واحد تبریز، استادیار گروه پاتوبیولوژی، تبریز، ایران.
3 - دانشگاه آزاد اسلامی، واحد شبستر، کارشناس ارشد گروه دامپزشکی، شبستر، ایران.

Received: 2013-08-19 Accepted : 2014-06-24 Published : 2014-05-22

Keywords: genetic diversity, Pasteurella multocida, Cattle and buffalo, Restriction Endonuclease Analysis,

Abstract :

   In order to increase information about the molecular diversity of Pasteurella multocida isolated from cattle and buffalo, 2 buffalo and 8 cattle isolates were investigated by Restriction Endonuclease Analysis (REA). REA was performed with Hha-I Endonuclease which established 2 distinct profiles: I and II.  Cattle and buffalo isolates fell into both REA profiles. Contrary to previous studies, the genetic diversity of the isolates was negligible. Considering the similarity of cattle and buffalo isolates is the present study, further studies witch larger samples should be carried out to investigate the possibility of inter-species transmission.     

References:

• Blackall, P.J. and Mifin, J.K. (2000). A review of Identification and typing of Pasteurella multocida Avian Pathology, 29: 271-287.


• Blackall, P.J., Pahoff, J.L., Marks, D., Fegan, N. and Morrow, C.J. (1995). Characterization of Pasteurella multocida isolates from fowl cholera outbreaks on seven turkey farms. Australian Veterinary Journal, 72(4): 135-138.


• Davies, R.L., MacCorquodale, R. and Reilly, S. (2004). Characterisation of bovine strains 2 of Pasteurella multocida and comparison with isolates of avian, ovine and porcine 3 origin. Veterinary Microbiology, 99: 145-158.


• Jabbari, A.R., Saharee, A. and Esmaily, F. (2002). Characterization of avian Pasteurella multocida isolates by protein profiles and Restrection endonuelase analysis chromosomal DNA. Archive of Razi Institute, 15(1): 143-159.


• Quinn, P.J., Markey, B.K., Carter, M.E., Donnelly, W.J. and Leonard, F.C. (2003). Veterinary Microbiology and Microbial Disease. U.K., London: Blackwell Publishing.


• Rudolph, K.M., Hunter D.L., Forey W.J., Cassirer E.F., Rimler R.B. and Ward A.C.S. (2003). Sharing of Pasteurella spp. Between Free-ranging Bighorn Sheep and Feral Goats. Journal of Wildlife Diseases, 39(4): 897-903.


• Shayegh, J., Atashpaz, S., Zahraei Salehi, T. and Hejazi, M.S. (2010a). Potential of Pasteurella multocida Isolated from Healthy and Diseased Cattle and Buffaloes in Induction of Diseases. Bulletin of the Veterinary Institute in Pulawy, 54: 299-304.


• Shayegh, J., Parvizi, M. and Hejazi, M.S. (2010b). Diversity of Caprine and Ovine Pasteurella multocida Isolates Based on 16s rRNA Gene Sequencing. Iranian Journal of Veterinary Research, 11(4): 373-377.


• Shayegh, J., Mikaili, P., Dolgari Sharaf, J., Kamani, J. and Beheshti, R. (2011). Restriction endonuclease analysis: isolation and identification of ovine and caprine Pasteurella multocida. Research Opinions in Animal and Veterinary Sciences, 1(9): 611-613.


• Weiser, G.C., DeLong, W.J., Paz, J.L., Shafii, B., Price, W.J. and Ward, A.C. (2003). Characterization of Pasteurella multocida associated with pneumonia in bighorn sheep. Journal of Wild Animal Disease, 39: 536-544.