Seroprevalence of Leptospira interrogans infection in horses from some horse clubs in Tehran by Microscopic Agglutination Test (MAT)
Subject Areas :
Veterinary Clinical Pathology
mohammadrahim Haji Hajikolaei
1
,
alireza Nafisi Mozaffar
2
,
samad Lotfollazadeh
3
,
masoud Ghorbanpour
4
,
gholamreza Abdollapour
5
1 - استاد ﮔﺮوه ﻋﻠﻮم درﻣﺎﻧﮕﺎﻫﻲ، داﻧﺸﻜﺪه داﻣﭙﺰﺷﻜﻲ، داﻧﺸﮕﺎه ﺷﻬﻴﺪ چمران اهواز، اهواز، ایران.
2 - داﻧﺶآﻣﻮﺧﺘﻪ داﻧﺸﻜﺪه داﻣﭙﺰﺷﻜﻲ، داﻧﺸﮕﺎه ﺷﻬﻴﺪ ﭼﻤﺮان اهواز، اهواز، ایران.
3 - دانشیار ﮔﺮوه ﻋﻠﻮم درﻣﺎﻧﮕﺎﻫﻲ، داﻧﺸﻜﺪه داﻣﭙﺰﺷﻜﻲ، داﻧﺸﮕﺎه تهران، تهران، ایران.
4 - استاد گروه پاتوبیولوی، داﻧﺸﻜﺪه داﻣﭙﺰﺷﻜﻲ، داﻧﺸﮕﺎه ﺷﻬﻴﺪ چمران اهواز، اهواز، ایران.
5 - دانشیار ﮔﺮوه ﻋﻠﻮم درﻣﺎﻧﮕﺎﻫﻲ، داﻧﺸﻜﺪه داﻣﭙﺰﺷﻜﻲ، داﻧﺸﮕﺎه تهران، تهران، ایران.
Received: 2015-11-09
Accepted : 2016-01-30
Published : 2016-01-21
Keywords:
horse,
Tehran,
seroprevalence,
MAT,
Leptospira interrogans,
Abstract :
In order to evaluate the seroprevalence of Leptospira interrogans infection in horses, blood samples were taken from 152 horses from 7 horse clubs in Tehran. Serum samples were examined using a microscopic agglutination test to detect the presence of antibodies against five live serotypes of Leptospira interrogans (grippotyphosa, pomona, icterohaemorrhagiae, canicula and hardjo). Of the tested samples, 23 horses (15/13%) were positive to one or more serotypes. Titer levels ranged from 1:100 to 1:200. Icterohaemorrhagiae (44/44%) was the most frequently detected serovar followed in descending order by gripotyphosa (29/62%), canicula (22/22%), pomona (3/7%) and serotype hardjo was negative. Statistical analysis using the chi-squared test showed there was no significant correlation differences between Leptospira interrogans infection and factors such as sex and age. The serum titers of infected horses ranged from 1:100 (n=19) to 1:200 (n=78). These results suggest that the icterohaemorrhagiae serovar may be the most prevalent serovar in the horse population Tehran.
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