Histometric and histopathologic study of the effects of celery and spearmint essence on the healing of experimental skin surgery in the rat

Subject Areas : Veterinary Clinical Pathology

Ali Rezaie ¹ , داریوش Mohajeri ² , بابک Rostam zadeh dehkharghani ³ , مسعود Taghi zadeh jahed ⁴ , صمد Mohammad Nejad-daryani ⁵

1 - Department of Clinical Sciences, Faculty of Veterinary Medicine, Islamic Azad University, Tabriz Branch, Tabriz, Iran
2 - Department of Pathobiology, Faculty of Veterinary Medicine, Islamic Azad University, Tabriz Branch, Tabriz, Iran
3 - Graduate of Veterinary Medicine, I Islamic Azad University, Tabriz Branch, Tabriz, Iran
4 - Member of young Researchers Club, Islamic Azad University, Tabriz Branch, Tabriz, Iran
5 - Ph.D student of Pharmacology, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran

Received: 2008-01-05 Accepted : 2008-05-19 Published : 2008-02-20

Keywords: Cerely, spearmint, surgical truma, repairment,

Abstract :

     In this study, histometrical and histopathological effects of celery and spearmint essences which were mixed as lotion were evaluated on the process of wound healing following surgical skin trauma in the rat. Forty five female Wistar rats were selected and after anesthesia with xylazine and ketamine, four full thickness wounds were created on their dorsum using number 7 biopsy punch. The rats were then randomly divided to three equal groups and numbered. In group one the herbal lotion was administered at the lower dose of 2ml /cm2 twice a day, in group two the higher dose was administered at 2ml /cm2 four times a day while group three or the control group received placebo (distilled water). The treatment continued for 21 days during which daily digital images were taken from the wounds and the wound area calculated using digital image analysis software. Also on days 0, 3, 7, 14 and 21 after trauma, the wounds were biopsied for histopathological evaluations (3 rats/group) and these rats were omitted from the rest of the study. Histometrical and histopathological findings indicated the stimulatory effect of the herbal lotion on wound healing during the epithelialization phase and this stimulatory effect was more effective and prominent in the treatment group which received the lower dose and the results were statistically significant (p<0.01).  

References:

1. حاج هاشمی، و. (1381): نشریة واحد تحقیق و توسعة شرکت باریج اسانس شماره‌های 7و8، صفحات: 34-30.


2. زرگری، ع. (1376): گیاهان دارویی، جلد دوم، چاپ ششم، انتشارات دانشگاه تهران، صفحات: 482-476.


3. زرگری، ع. (1376): گیاهان دارویی، جلد چهارم، چاپ ششم، انتشارات دانشگاه تهران، صفحات: 30-1.


4. صمام شریعت، ه. (1382): تجزیه و شناسایی مواد دارویی گیاهان، چاپ دوم، نشر فرهنگ معاصر، صفحات: 90-51.


5. قهرمان، ا. (1373): سیستماتیک گیاهی، جلد دوم، چاپ اول، مرکز نشر دانشگاهی، صفحه: 726.                        


6. قهرمان، ا. (1362): فلور ایران، جلد نهم، چاپ اول، مؤسسه تحقیقات جنگل‌ها و مراتع، صفحات: 150-120.


7. محمد نژاد، ص. (1386): بررسی اثرات هیستومتریک وهیستوپاتولوژیکی مصرف عصاره گیاهی Pelargonium roseum در مقایسه با Phenytoin Sodium بعد از ترومای جراحی در پوست رت، پایان‌نامه جهت اخذ دکترای دامپزشکی، دانشگاه آزاد اسلامی واحد تبریز.


8. مظفریان، و. (1375): فرهنگ‌نامة گیاهان ایران، جلد اول،  چاپ دوم، صفحات: 50-45.                                                                                                     


9. میرحیدر، ح. (1372): معارف گیاهی، جلد اول، چاپ دوم، نشر فرهنگ اسلامی، صفحه: 215.


10. Amann, R. and Peskar, B. (2002): Molecular Pharmacology. Saunders, Philadelphia, pp: 121-124.


11. Andrew, C. (1996): The Encyclopedia of Medicinal Plants. Saunders, Philadelphia, 61: 768-79.


12. Beck, A. (2003): Influence of herbal extract of Echinacea on fracture healing. Arch. Orthop. Trauma Surg., 123(7): 327-32.


13. Carola, N. and Linda Anderson, J. (1996): Danid philipson, Herbal Medicines, 65:102-190.


14. Duke, J.A. (1989): CRC Handbook of Medicinal Herbs. Boca Raton: CRC press Inc., 12: 45-6.


15. Kimball, O. and Horan T.N. (1993): The use of dilantine in the treatment of epilepsy. Ann Intern Med., 13: 787-93.


16. Kolbert, G. (1987): Oral diphenylhydantoin in corneal wound healing in the rabbit. Am. J. Opthamol., 66: 736-8.        


17. Wichtl, M. (1994): Herbal Drugs and Phytopharmaceuticals. Translated by: Bisset N.G. Boca Raton: CRC press, 2, 81-2.


18. Xu, H.Q. and Hao, H.P. (2004): Effect of iridoid total glycoside from cornus officinalis on prevention of glomerular overexpression of transforming growth factor beta 1 and matrixes an experimental diabetes model. Biol. Pharm. Bull., 27(7): 1014-8.