Effect of vitamin E supplementation on serumic levels of lipids and lipoproteins in cholesterol-fed male rat

Subject Areas : Veterinary Clinical Pathology

M.H Khayat Nouri ¹ , محمد Mashayekhi ² , رضا Jafari ³ , M.R Nasirzade ⁴ , جعفر Rahmani ⁵

1 - Department of Basic Science, Faculty of Veterinary Medicine, Islamic Azad University, Tabriz Branch, Tabriz, Iran
2 - گروه علوم درمانگاهی، دانشکده دامپزشکی، دانشگاه آزاد اسلامی واحد تبریز، تبریز، ایران
3 - Graduate of Veterinary Medicine, Islamic Azad University, Tabriz Branch, Tabriz, Iran
4 - Department of Basic Science, Faculty of Veterinary Medicine, Islamic Azad University, Tabriz Branch, Tabriz, Iran
5 - Department of Clinical Science, Faculty of Veterinary Medicine, Islamic Azad University, Tabriz Branch, Tabriz, Iran

Received: 2008-10-30 Accepted : 2009-01-09 Published : 2008-11-21

Keywords: Hypercholesterolemia, vitamin E supplementation, Male rat,

Abstract :

     Hypercholesterolemia is one of the risk factors of cardiovascular diseases. High blood cholesterol affects the general health and increases the mortality rate of cardiovascular diseases. High levels of cholesterol in the diet increases LDL levels and decreases the activity of LDL receptors in the liver. Oxidation of vascular LDL lipoproteins increases the development of atherosclerosis. Previous studies have indicated that consumption of antioxidants decreases hypercholesterolemia. This study evaluates the effect of vitamin E supplementation on blood lipid levels in high cholesterol-fed rats. In this experimental study, three groups of male rats (n=10 for each group) were used. The control group received basic diet and one of the other two groups received a diet containing one percent cholesterol and while the other received the same diet plus vitamin E supplement (2500 IU/kg in dry matter of the diet) for one month. After determining the values of TC, LDL, VLDL, HDL and TG the results indicated that in rats fed with 1% cholesterol apart from HDL and VLDL the other lipids had increased significantly compared with the control group (p<0.01). Supplementation with vitamin E significantly decreased the TC and LDL levels in comparison with the 1% cholesterol fed group (p<0.01). This effect of vitamin E may be related to its antioxidant and inhibitory effects on lipoprotein synthesis pathway. The validity of these points in humans needs further investigations.  

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