Comparison of commercial ELISA kit with serum neutralization (SN) in diagnosis of BHV-1 infections in water buffalo (Buballous buballis)
Subject Areas :
Veterinary Clinical Pathology
neda arjmandi
1
,
mohammad rahim hajikolayei
2
,
masoudreza seyfi abad shapouri
3
,
maryam dagheri
4
1 - داﻧﺶ آﻣﻮﺧﺘﻪ داﻧﺸﻜﺪه داﻣﭙﺰﺷﻜﻲ، داﻧﺸﮕﺎه ﺷﻬﻴﺪ ﭼﻤﺮان اهواز، اهواز، ایران.
2 - استاد ﮔﺮوه ﻋﻠﻮم درﻣﺎﻧﮕﺎﻫﻲ، داﻧﺸﻜﺪه داﻣﭙﺰﺷﻜﻲ، داﻧﺸﮕﺎه ﺷﻬﻴﺪ چمران اهواز، اهواز، ایران.
3 - استاد ﮔﺮوه ﻋﻠﻮم درﻣﺎﻧﮕﺎﻫﻲ، داﻧﺸﻜﺪه داﻣﭙﺰﺷﻜﻲ، داﻧﺸﮕﺎه ﺷﻬﻴﺪ چمران اهواز، اهواز، ایران.
4 - کارشناس ﮔﺮوه ﻋﻠﻮم درﻣﺎﻧﮕﺎﻫﻲ، داﻧﺸﻜﺪه داﻣﭙﺰﺷﻜﻲ، داﻧﺸﮕﺎه ﺷﻬﻴﺪ چمران اهواز، اهواز، ایران.
Received: 2015-09-04
Accepted : 2015-12-21
Published : 2015-11-22
Keywords:
Buffalo,
Elisa,
Serum neutralization,
Bovine Herpesvirus-1,
Abstract :
In order to compare commercial ELISA kit with serum neutralization (SN) in diagnosis of BHV-1 infection, blood samples were taken from 150 (100 male, 50 female) slaughtered water buffalo (Buballous buballis) at Ahvaz abattoir. Sera were tested by commercial ELISA kit and SN to determine antibodies against BHV-1. Out of 150 buffaloes, prevalence of infection with BHV-1 in ELISA and SN was 54% (81 samples) and 58/7% (88 samples) respectively. Statistical analysis showed there was no significant difference between ELISA and SN for detection of antibodies to BHV-1 in sera of water buffalo.
References:
حاجی حاجیکلایی، م.ر. و صیفی آباد شاپوری، م.ر. (1386).بررسی سرواپیدمیولوژی آلودگی با هرپس ویروس تیپ 1 (BHV-1) در گاوهای اهواز. مجله دامپزشکی ایران، دوره 2، شماره 2، صفحات: 32-23.
حاجی حاجیکلایی، م.ر. و صیفی آباد شاپوری، م.ر. (1385). فراوانی حضور آنتیبادی برعلیه اسهال ویروسی گاو و هرپس ویروس تیپ 1 در گاومیشهای کشتار شده در کشتارگاه اهواز. طرح تحقیقاتی شماره 641.
حضرتی، ح. (1355). هرپس ویروس های گاوی و نقش بیماری زایی آنها. نشریه شماره 22 سازمان تحقیقات کشاورزی انستیتو رازی.
کارگر، م.ر.، بکائی، س. و شکوه، م. (1381). بررسی میزان شیوع پادتنهای ضد ویروسهای BLV, BH4 , IBR, BVD, PI3 در گاومیش های مستقر در مرکز تهیه اسپرم در ارومیه. پژوهش و سازندگی، شماره 2، جلد 15، صفحات 23-16.
مامی، ف.، حاجی حاجیکلایی، م.ر. و صیفی آباد شاپوری، م.ر. (1394). مقایسه کیت تجاری الیزا با آزمایش خنثی سازی ویروس جهت تعیین آنتی بادی ضد ویروس اسهال ویروسی گاو در گاومیش. مجله دامپزشکی ایران. در دست چاپ.
همتزاده، ف.، ممتاز، ح.، تاج بخش، ا. و صفری، ح. (1381). بررسی سرولوژی آلودگی به ویروس IBR در گاوداری های استان چهارمهال بختیاری. پژوهشی و سازندگی، شماره 2، جلد 15، صفحات: 43-38.
Amoroso, M.G., Corrado, F., Carlo, E. De., Lucibelli, M.G., Martucciello, A., Guarino, A., et al. (2013). Bubaline herpesvirus 1 associated with abortion in a Mediterranean water buffalo. Research Veterinary Science 94: 813-816.
Castrucci, G., Ferrari, M., Osburn, B.I., Frigeri, F., Barreca, F., Tagliati, S., et al. (1996). Further investigations on the efficacy of a nonspecific defense inducer evaluated in calves exposed to infectious bovine-rhinotracheitis virus. Comparative Immunology, Microbiology and Infectious Disease, 2(21): 155-163.
Albayrak, H., Ozan, E., Beyhan, Y.M., Kurt, M. and Kilicoglu, Y. (2012). A serological Investigation of Some Aetiological Agents Associated with Abortion in Domestic Water Buffalo (Bubalus bubalis Linneaus, 1758) in Samsun Province of Northern. Atatürk Üniversitesi Veteriner Bilimleri Dergisi, 7(3): 155-160
Carvalho, O.S., Gonzaga, L.N.R., Albuquerque, A.S., Bezerra, D.C. and Chves, N.P. (2015). Ocuurence of Brucella abortus, Leptospira interrogans and bovine herpesvirus type 1 in buffalo (Bubalus bubalis) herd under extensive breeding system. African Journal of Microbiology Research, 9(9): 593-603.
Ferankenak, F.P. (1997). Probability of detecting antibodies to BHV-1 in bulk milk after production of a positive animal on to negative farm. Journal of Veterinary Record, 140: 90-92.
Karshoek, M.J., Rijsew, J.K. and Vanairschat, J.T. (1996). Persistent of antibodies against BHV-1 and Virus reactivation two to three years after infection. Veterinary Microbiology, 53: 103-110.
Kampa, J. (2006). Epidemiology of Bovine viral diarrhea virus and bovine herpesirus type 1 Infections in dairycattle herds, evidence of self-clearance and detection of infection with a new atypical pestivirus. Doctoral thesis Swedish University of Agricultural Sciences Uppsala, pp: 17-21
Lucas, M.H., Westcott, D.G. and Edwards, S. (1986). Immunofluorescence and cell culture techniques in the diagnosis of viral infection of abortive fetus. Journal of Veterinary Record, 114: 243-242.
McDermott, J.J., Kadohira, M., Ocallaghan, C.J. and Shoukri, M. (1997). A comparison of different Models for assessing Variations in the sero-prevalence of infectious bovine rhinotracheitis by farm, area and district in Kenya. Preventive Veterinary Medicine, 32: 219-234.
Motha, M.X.Y and Hansen, M.F. (1998). Prevalence of IBR (infectious bovine rhinotracheitis), PI3 (parainfluenza type 3), BRS (bovine respiratory syncytial) and BCV (bovine coronavirus) infections in the dairy cattle population of New Zealand. New- Zealand Veterinary Journal, 46(6): 239-240.
Riegel, C.A., Ayers, V.K. and Collins, J.K. (1987). Rapid, sensitive, competitive serologic enzyme-linked immunosorbent assay for detecting serum antibodies to bovine herpesvirus type 1. Journal of Clinical Microbiology, 25(12): 2418-2421.
Robert, F.K. (2001). Viral Disease of Cattle. Second edition. USA: Iowa State, University Press, pp: 113-126, 159-170.
Rodostits, O.M., Gay, C.C., Hinchcliff, K.W., and Constable, P.D. (2007). Veterinary Medicine. 9th ed., London: W.B. Saunders, pp: 1085-1105.
Scicluna, M.T., Saralli, G., Bruni, G., Sala, M., Cocumelli, C., Caciolo, D., et al. (2007). Epidemiological situation of Herpesvirus infections in buffalo herds: Bubaline Herpesvirus1 or Bovine Herpesvirus1. Italian Journal of Animal Science, 6: 845-849.
Smith, B.P. (2009). Large Animal Internal Medicine. 2nd ed., London: Mosby, 635-636, 809-814.
Tautz, N., Meyers, G. and Thiel, H.J. (1998). Pathogenesis of mucosal disease a deadly disease of cattle caused by a pestiviruse. Clinical and Diagnostic Virology, 10: 121-127.
Tekes, L., Markos, B., Kecskemeti, S., Meheshalvi, J., Mate, Z. and Kudron, E. (1999). Prevalence of bovine herpesvirus 1(BHV-1) infection in Hungarian cattle herds. Acta Veterinary Hungarica, 47(3): 303-309.
Yossef, N.M.A. (1997). Prevalence of antibodres to bovine res piratory syncytial virus, infectious bovine rhinotracheitis and parainflaenza-3 in Cattle and buffalo calves. Egyptian Journal of Agriculture Research, 75(4): 1135-1146.
FAO. (1977). Animal Production and Health, the Water Buffalo, 4: 109-111.