Evaluation of the serumic levels of creatinine, urea and uric acid in rainbow trouts (Oncorhyncus mykiss) affected by nephrocalsinosis

Subject Areas : Veterinary Clinical Pathology

جاوید Mortazavi Tabrizi ¹ , فرهنگ Sasani ² , بهیار Jalali Jafari ³

1 - Department of Aquatic Diseases, Faculty of Veterinary Medicine, Islamic Azad University of Tabriz, Tabriz, Iran
2 - Department of Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
3 - Department of Aquatic Diseases, Faculty of Veterinary Medicine, Islamic Azad University, Science & Research Campus, Tehran, Iran

Received: 2007-11-09 Accepted : 2008-02-07 Published : 2007-11-22

Keywords: Rainbow trout, Urea, Creatinine, Uric acid, Nephrocalcinosis,

Abstract :

      In order to investigate the alternation in serumic levels of creatinine, urea and uric acid in rainbow trouts (Oncorhyncus mykiss) affected by nephrocalsinosis, samples were taken during the winter of 1385 from three fish farms with different water sources of river, spring and well water recirculation system. Five samples were taken from each of the 8 different weight groups and a total of 120 specimens were collected randomly. After collecting the specimens, blood and renal tissue sampling was performed on all the fish with the tissue samples fixed in 10% buffered formalin and the separated sera stored at -200C for further evaluations. Renal Samples were transferred to the pathology laboratory and tissue sections were prepared and stained with hematoxylin and eosin. From the 120 collected specimens, 6 were affected by nephrocalcinosis with histopathologic evidence of severe dilation of renal tubules, presence of dense basophilic materials inside the dilated tubules and hyaline cast formation in some renal tubules. Amongst the 6 cases with nephrocalcinosis, 3(2.5%) were from the farm with well water recirculation system, 2 (1.66%) from the farm with river water supply and 1 (0.83%) from the farm which used the spring water source.  Evaluation of the amounts of urea, creatinine and uric acid indicated that there was a significant difference in mean creatinine levels between different weight groups (P< 0.05). In conclusion, according to the results of this study there is only a significant statistical difference in the amount of creatinine between healthy fish and those affected by nephrocalcinosis in each farm.  

References:

1. شاهسونی، د. و موثق، ا. (1381): آسیب‌شناسی سیستماتیک ماهی، انتشارات دانشگاه فردوسی مشهد، صفحات: 109-138.


2. مجابی، ع.، نظیفی حبیب آبادی، س. و صافی، ش. (1379): بیوشیمی درمانگاهی دامپزشکی، انتشارات نوربخش، تهران، صفحات: 385-393.


3. Chun-Yao, Ch. (2001): Nephrocalcinosis in Nile tilapia from a recirculation aquaculture system, Case Report. Journal of Aquatic Animal Health. Vol. 13, pp: 368-372.


4. Bruno, D. and Pope, T. (1996): A Colour Atlas of Salmonid Disease. Academic press. Harcoart Brace, London, pp: 145.


5. Coffey, I. (2003): Nephrocalcinosis in Tilapia from a recirculation production facility. CornellUniversity Seminar. Available at: http://hdl.handle.net/1813/2562.


6. Colt, J. and Orwics, K. (1991): Modeling production capacity of aquatic culture systems under fresh water conditions. Acuaculture Engneering. 10: 1-29.


7. Eddy, F.B., Lomholt, J.P., Weber, R.E. and Johansen, K. (1977): Blood respiratory properties of rainbow trout kept in water of high CO₂ tension. Journal of Experience Biology. 67: 37-47.


8. Fivelstad, S., Bergheim, A., Holland, P.M. and Fjermedal, A.B. (2004): Water flow requirements in the intessive production of Atlantic salmon parr-smolt at two salinity levels. Aquaculture. 231: 263-277.


9. Fivelstad, S., Olsen, A., Kloften, H., Ski, H. and Steffanson, S. (1999): Effects of carbon dioxide on Atlantic salmons smolts at constant pH in bicarbonate rich freshwater. Aquaculture. 178: 171-187.


10. Foss, A., Rosnes, B.A. and Oiestad, V. (2003): Graded environmental hypercapnia in juvenile spotted wolfish (Anarhincus minor olafsen). Effects on growth, food conversion efficiency and Nephrocalcinosis. Aquaculture. 220: 607-617.


11. Hicks, B.D., Hilton, W. and Ferguson, H.W. (1984): Influence of dietary selenium on the occurrence of nephrocalcinosis in the rainbow trout. Journal of Fish Disease. 7: 379-389.


12. Hilton, J.W. and Hodson, P.V. (1983): Effect of increased dietary carbohydrate on selenium metabolism and toxicity in rainbow trout. Journal of Nutrition. 113: 1241-1248.


13. Jelmert, A., Sethom, R. and Meerent, V. (1995): Nephrocalcinosis associatied with mass mortality in cultured Atlantic halibut, Hpposglossus hippoglossus, larvae. Journal of Fish Disease. 18: 365-369.


14. Knoy, D., Cowey, C.B. and Andron, J.W. (1981): Studies on the nutrition of salmonid fish. The mangnesium requirements of rainbow trout. Journal of Nutrition. 45: 137-148.


15. Lloyd, R. and Swift, W.R. (1967): Effect of high concentration of carbon dioxide on the ionic composition of rainbow trout blood. Nature. 216: 1341-1342.


16. Martens, L.G. and Witten, P.E. (2006): Impact of high water carbon dioxide levels on Atlantic salmon smolts: Effects on fish performance, vertebrate composition and structure.  Aquaculture. 261: 80-88.


17. Roberts, R. (2001): Fish Pathology. Baillieve tindall, London, pp: 256


18. Smart, G.R. (1979): Nephrocalcinosis in rainbow trout; the effect of exposure tolerated CO₂ concentration. Journal of Fish Disease. 2: 279-289.


19. Smart, G.R. (1981): Aspects of water quality producing stress in intensive fish culture. Pickering, A.D. Stress and fish. Academic press, London, pp: 277-289.